Document Detail

Monitoring and robust induction of nephrogenic intermediate mesoderm from human pluripotent stem cells.
MedLine Citation:
PMID:  23340407     Owner:  NLM     Status:  MEDLINE    
A method for stimulating the differentiation of human pluripotent stem cells into kidney lineages remains to be developed. Most cells in kidney are derived from an embryonic germ layer known as intermediate mesoderm. Here we show the establishment of an efficient system of homologous recombination in human pluripotent stem cells by means of bacterial artificial chromosome-based vectors and single-nucleotide polymorphism array-based detection. This system allowed us to generate human-induced pluripotent stem cell lines containing green fluorescence protein knocked into OSR1, a specific intermediate mesoderm marker. We have also established a robust induction protocol for intermediate mesoderm, which produces up to 90% OSR1(+) cells. These human intermediate mesoderm cells can differentiate into multiple cell types of intermediate mesoderm-derived organs in vitro and in vivo, thereby supplying a useful system to elucidate the mechanisms of intermediate mesoderm development and potentially providing a cell source for regenerative therapies of the kidney.
Shin-Ichi Mae; Akemi Shono; Fumihiko Shiota; Tetsuhiko Yasuno; Masatoshi Kajiwara; Nanaka Gotoda-Nishimura; Sayaka Arai; Aiko Sato-Otubo; Taro Toyoda; Kazutoshi Takahashi; Naoki Nakayama; Chad A Cowan; Takashi Aoi; Seishi Ogawa; Andrew P McMahon; Shinya Yamanaka; Kenji Osafune
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nature communications     Volume:  4     ISSN:  2041-1723     ISO Abbreviation:  Nat Commun     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-23     Completed Date:  2013-06-20     Revised Date:  2014-06-26    
Medline Journal Info:
Nlm Unique ID:  101528555     Medline TA:  Nat Commun     Country:  England    
Other Details:
Languages:  eng     Pagination:  1367     Citation Subset:  IM    
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MeSH Terms
Cell Differentiation
Cell Line
Embryoid Bodies / cytology
Embryonic Stem Cells / cytology
Flow Cytometry
Gene Dosage / genetics
Gene Expression Regulation, Developmental
Gene Knock-In Techniques
Gene Targeting
Genetic Loci / genetics
Green Fluorescent Proteins / metabolism
Induced Pluripotent Stem Cells / cytology*,  metabolism
Kidney / cytology,  embryology*,  metabolism
Mesoderm / cytology*,  embryology*
Polymorphism, Single Nucleotide / genetics
Protein-Serine-Threonine Kinases / metabolism
Time Factors
Grant Support
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins; EC kinase, mouse; EC Kinases

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