Document Detail


Monitoring of renal venous PO2 and kidney oxygen consumption in rats by a near-infrared phosphorescence lifetime technique.
MedLine Citation:
PMID:  18184739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Renal oxygen consumption (Vo(2,ren)) is an important parameter that has been shown to be influenced by various pathophysiological circumstances. Vo(2,ren) has to be repeatedly measured during an experiment to gain insight in the dynamics of (dys)regulation of oxygen metabolism. In small animals, the classical approach of blood gas analysis of arterial and venous blood samples is only limitedly applicable due to fragile vessels and a low circulating blood volume. We present a phosphorescence lifetime technique that allows near-continuous measurement of renal venous Po(2) (vPo(2)) and Vo(2,ren) in rats. The technique does not rely on penetration of the blood vessel, but uses a small reflection probe. This probe is placed in close proximity to the renal vein for detection of the oxygen-dependent phosphorescence of the injected water-soluble near-infrared phosphor Oxyphor G2. The technique was calibrated in vitro and the calibration constants were validated in vivo in anesthetized and mechanically ventilated male Wistar rats. The hemoglobin saturation curve and its pH dependency were determined for calculation of renal venous oxygen content. The phosphorescence technique was in good agreement with blood gas analysis of renal venous blood samples, for both Po(2) and hemoglobin saturation. To demonstrate its feasibility in practice, the technique was used in four rats during endotoxin infusion (10 mg x kg(-1) x h(-1) during 1 h). Renal vPo(2) reduced by 40% upon reduction in oxygen delivery to 30% of baseline, but Vo(2) remained unchanged. This study documents the feasibility of near-continuous, nondestructive measurement of renal vPo(2) and Vo(2) by oxygen-dependent quenching of phosphorescence.
Authors:
Egbert G Mik; Tanja Johannes; Can Ince
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Publication Detail:
Type:  Evaluation Studies; Journal Article     Date:  2008-01-09
Journal Detail:
Title:  American journal of physiology. Renal physiology     Volume:  294     ISSN:  1931-857X     ISO Abbreviation:  Am. J. Physiol. Renal Physiol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-28     Completed Date:  2008-05-08     Revised Date:  2011-04-28    
Medline Journal Info:
Nlm Unique ID:  100901990     Medline TA:  Am J Physiol Renal Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  F676-81     Citation Subset:  IM    
Affiliation:
Department of Physiology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. e.mik@erasmusmc.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Hemoglobins / metabolism
Kidney / metabolism
Luminescent Measurements / instrumentation,  methods*
Male
Metalloporphyrins / diagnostic use*
Oxygen / blood*
Oxygen Consumption / physiology*
Rats
Rats, Wistar
Renal Veins / metabolism*
Spectroscopy, Near-Infrared
Chemical
Reg. No./Substance:
0/Hemoglobins; 0/Metalloporphyrins; 0/oxyphor G2; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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