| Monitoring the progression of the in vitro selection of nucleic acid aptamers by denaturing high-performance liquid chromatography. | |
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MedLine Citation:
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PMID: 17994225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monitoring of in-vitro selection experiments is crucial in evaluation of the success and outcome of such approaches. Furthermore, monitoring running in parallel with the selection procedure enables early intervention and adjustment of stringency to achieve the desired activities of the selected nucleic acid species. Here we describe the use of a non-radioactive method that enables monitoring of a SELEX procedure on the basis of sequence diversity. We employ denaturing HPLC and describe for the first time an experimental set-up that is useful both for analysis of the progression of in-vitro selection experiments and for separation of distinct aptamer sequences. |
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Authors:
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Jens Müller; Osman El-Maarri; Johannes Oldenburg; Bernd Pötzsch; Günter Mayer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-11-10 |
Journal Detail:
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Title: Analytical and bioanalytical chemistry Volume: 390 ISSN: 1618-2650 ISO Abbreviation: Anal Bioanal Chem Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-05 Completed Date: 2008-03-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101134327 Medline TA: Anal Bioanal Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 1033-7 Citation Subset: IM |
Affiliation:
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Institute of Experimental Haematology and Transfusion Medicine, University of Bonn, Sigmund-Freud-Str. 25, 53127, Bonn, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide* Base Sequence Chromatography, High Pressure Liquid / methods* DNA Primers DNA, Single-Stranded / chemistry Polymerase Chain Reaction |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 0/DNA Primers; 0/DNA, Single-Stranded |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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