| Monitoring of polyomavirus BK viruria in bone marrow transplantation patients by DNA hybridization assay and by polymerase chain reaction: an approach to assess the relationship between BK viruria and hemorrhagic cystitis. | |
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MedLine Citation:
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PMID: 7994238 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An association between long-lasting hemorrhagic cystitis (HC) in bone marrow transplantation (BMT) patients and viral infections, mostly with reactivation of the human polyomavirus BK (BKV), is suggested by several previous reports. We have carried out a prospective study in 55 (30 allogeneic, 24 autologous, 1 syngeneic) BMT recipients with the aim of evaluating the role of BKV in this frequent complication after BMT. To overcome the well known difficulties in BK virus isolation in cell cultures, a DNA hybridization assay and the polymerase chain reaction (PCR) were used for the detection and monitoring of viral urinary shedding, respectively. The presence of human polyomavirus JC and adenovirus DNA was also sought in urine specimens. BK viruria was demonstrated in 52.7% of patients (in 53.3% allogeneic and in 54.1% autologous BMT), whereas JCV and adenovirus were seldom present. Seven cases of HC (20% in allogeneic and 4% in autologous BMT) occurred and in all cases the clinical event was associated with BKV urinary shedding. This study suggests that BKV infection alone does not invariably lead to HC in BMT patients; for HC to occur the presence of other, at present unidentified, factors seems to be necessary. |
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Authors:
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A Azzi; R Fanci; A Bosi; S Ciappi; K Zakrzewska; R de Santis; D Laszlo; S Guidi; R Saccardi; A M Vannucchi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bone marrow transplantation Volume: 14 ISSN: 0268-3369 ISO Abbreviation: Bone Marrow Transplant. Publication Date: 1994 Aug |
Date Detail:
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Created Date: 1995-01-13 Completed Date: 1995-01-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8702459 Medline TA: Bone Marrow Transplant Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 235-40 Citation Subset: IM |
Affiliation:
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Institute of Microbiology, University of Florence, Italy. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult BK Virus / genetics* Base Sequence Bone Marrow Transplantation / adverse effects* Cystitis / etiology* DNA, Viral / urine* Female Hemorrhage / etiology Humans Male Middle Aged Molecular Sequence Data Nucleic Acid Hybridization Polymerase Chain Reaction Polyomavirus Infections / diagnosis* Prospective Studies Tumor Virus Infections / diagnosis* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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