| Monitoring neovascularization of intraportal islet grafts by dynamic contrast enhanced magnetic resonance imaging. | |
| | |
MedLine Citation:
|
PMID: 21099279 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Fifteen thousand youths are diagnosed yearly with type 1 diabetes mellitus. Pancreatic islet transplantation has been shown clinically to provide short-term (~1 year) insulin independence. However, challenges associated with early vascularization of transplanted islet grafts and long-term islet survival remain. We utilized dynamic contrast enhanced magnetic resonance imaging (DCE MRI) to monitor neovascularization of islets transplanted into the right lobe of the liver in a syngeneic mouse model system. The left lobe received no islets and served as a control. DCE data were analyzed for temporal dynamics of contrast (gadolinium) extravasation and the results were fit to a Tofts two-compartment exchange model. We observed maximal right lobe enhancement at seven days post-transplantation. Histological examination up to 28 days was used to confirm imaging results. DCE-derived enhancement strongly correlated with immunohistochemical measures of neovascularization. To our knowledge, these results are the first to demonstrate using a FDA approved contrast agent that DCE MRI can effectively and non-invasively monitor the progression of angiogenesis in intraportal islet grafts. |
| | |
Authors:
|
Nathaniel Chan; Andre Obenaus; Annie Tan; Naoaki Sakata; John Mace; Ricardo Peverini; Richard Chinnock; Lawrence C Sowers; Eba Hathout |
Related Documents
:
|
12110719 - Quantification of flow dynamics in congenital heart disease: applications of velocity-e... 2967709 - Application of dynamic computed tomography to physiologic imaging of renal artery steno... 19820949 - Dynamic contrast-enhanced mr imaging to assess physiologic variations of myometrial per... 14571329 - Morphologic parameters of normal swallowing events using single-shot fast spin echo dyn... 9438509 - Dynamic contrast-enhanced magnetic resonance imaging in a model of splenic metastasis. 8367519 - A statistical model for the determination of the optimal metric in factor analysis of m... 11085669 - Giant congenital melanocytic nevus with underlying hypoplasia of the subcutaneous fat. 2031449 - Impact of magnetic resonance imaging (mri) on the epidemiology of ms. 6784559 - Simplified ct-guided stereotaxic biopsy. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Islets Volume: 1 ISSN: 1938-2022 ISO Abbreviation: Islets Publication Date: 2009 Nov-Dec |
Date Detail:
|
Created Date: 2010-11-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101495366 Medline TA: Islets Country: United States |
Other Details:
|
Languages: eng Pagination: 249-55 Citation Subset: IM |
Affiliation:
|
Islet Transplant Laboratory, Department of Pediatrics, Loma Linda University School of Medicine, Loma Linda, CA, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
1-R01-DK077541-03/DK/NIDDK NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Impaired insulin secretion in transgenic mice over-expressing calpastatin in pancreatic ?-cells.
Next Document: CXCL10- a path to ?-cell death.