Document Detail


Monitoring of morphology and physical properties of cultured cells using a micro camera and a quartz crystal with transparent indium tin oxide electrodes after injections of glutaraldehyde and trypsin.
MedLine Citation:
PMID:  18706321     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
For investigating the effects of chemical stimulation to cultured cells, we have developed a quartz crystal sensor system with a micro charge-coupled device (CCD) camera that enables microphotograph imaging simultaneously with quartz crystal measurement. Human hepatoma cell line (HepG2) cells were cultured on the quartz crystal through a collagen film. The electrode of the quartz crystal was made of indium tin oxide (ITO) transparent electrodes that enable to obtain a transparent mode photograph. Glutaraldehyde and trypsin were injected to the chamber of the cells, respectively. The response of the quartz crystal was monitored and microphotographs were recorded, and the resonance frequency and resonance resistance were analyzed with an F-R diagram that plotted the resonance frequency and resonance resistance. In the case of the glutaraldehyde injection, the cells responded in two steps that included the fast response of the cross-linking reaction and the successive internal change in the cells. In the case of the trypsin injection, the responses included two processes. In the first step, cell adhesion factors were cleaved and the cell structure became round, and in the next step, the cells were deposited on the quartz crystal surface and the surface of the cells was directly in contact with the quartz crystal surface.
Authors:
Hyen-Wook Kang; Kazumi Ida; Yuji Yamamoto; Hiroshi Muramatsu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-01
Journal Detail:
Title:  Analytica chimica acta     Volume:  624     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-18     Completed Date:  2008-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  154-61     Citation Subset:  IM    
Affiliation:
School of Bioscience and Biotechnology, Tokyo University of Technology, 1404-1 Katakura, Hachoji, Tokyo 192-0982, Japan.
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MeSH Terms
Descriptor/Qualifier:
Biosensing Techniques / methods*
Cell Adhesion / physiology
Cell Culture Techniques / methods*
Cell Line, Tumor
Edetic Acid / pharmacology
Extracellular Matrix / chemistry
Glutaral / pharmacology
Humans
Microelectrodes
Photography / instrumentation
Quartz / chemistry*
Tin Compounds / chemistry*
Trypsin / pharmacology
Chemical
Reg. No./Substance:
0/Tin Compounds; 111-30-8/Glutaral; 14808-60-7/Quartz; 60-00-4/Edetic Acid; 71243-84-0/indium tin oxide; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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