Document Detail

Monitoring in vivo changes in lung microstructure with ³He MRI in Sendai virus-infected mice.
MedLine Citation:
PMID:  22383505     Owner:  NLM     Status:  MEDLINE    
Recently, a Sendai virus (SeV) model of chronic obstructive lung disease has demonstrated an innate immune response in mouse airways that exhibits similarities to the chronic airway inflammation in human chronic obstructive pulmonary disease (COPD) and asthma, but the effect on distal lung parenchyma has not been investigated. The aim of our study is to image the time course and regional distribution of mouse lung microstructural changes in vivo after SeV infection. (1)H and (3)He diffusion magnetic resonance imaging (MRI) were successfully performed on five groups of C57BL/6J mice. (1)H MR images provided precise anatomical localization and lung volume measurements. (3)He lung morphometry was implemented to image and quantify mouse lung geometric microstructural parameters at different time points after SeV infection. (1)H MR images detected the SeV-induced pulmonary inflammation in vivo; spatially resolved maps of acinar airway radius R, alveolar depth h, and mean linear intercept Lm were generated from (3)He diffusion images. The morphometric parameters R and Lm in the infected group were indistinguishable from PBS-treated mice at day 21, increased slightly at day 49, and were increased with statistical significance at day 77 (p = 0.02). Increases in R and Lm of infected mice imply that there is a modest increase in alveolar duct radius distal to airway inflammation, particularly in the lung periphery, indicating airspace enlargement after virus infection. Our results indicate that (3)He lung morphometry has good sensitivity in quantifying small microstructural changes in the mouse lung and that the Sendai mouse model has the potential to be a valid murine model of COPD.
Wei Wang; Nguyet M Nguyen; Eugene Agapov; Michael J Holtzman; Jason C Woods
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-03-01
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  112     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-02     Completed Date:  2012-08-27     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1593-9     Citation Subset:  IM    
Department of Physics, Washington Univ. Box 8131, Dept. of Radiology, 510 S. Kingshighway, St. Louis, MO 63110, USA.
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MeSH Terms
Diffusion Magnetic Resonance Imaging*
Disease Models, Animal
Helium / diagnostic use*
Lung / pathology*,  virology
Lung Volume Measurements
Mice, Inbred C57BL
Predictive Value of Tests
Pulmonary Alveoli / pathology,  virology
Pulmonary Disease, Chronic Obstructive / pathology*,  virology
Respirovirus Infections / pathology*,  virology
Sendai virus / pathogenicity*
Sensitivity and Specificity
Time Factors
Grant Support
Reg. No./Substance:
0/Isotopes; 7440-59-7/Helium

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