Document Detail


Monitoring impedance changes associated with motility and mitosis of a single cell.
MedLine Citation:
PMID:  20676434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present a device enabling impedance measurements that probe the motility and mitosis of a single adherent cell in a controlled way. The micrometre-sized electrodes are designed for adhesion of an isolated cell and enhanced sensitivity to cell motion. The electrode surface is switched electro-chemically to favour cell adhesion, and single cells are attracted to the electrode using positive dielectrophoresis. Periods of linear variation in impedance with time correspond to the motility of a single cell adherent to the surface estimated at 0.6 μm h(-1). In the course of our study we observed the impedance changes associated with mitosis of a single cell. Electrical measurements, carried out concomitantly with optical observations, revealed three phases, prophase, metaphase and anaphase in the time variation of the impedance during cell division. Maximal impedance was observed at metaphase with a 20% increase of the impedance. We argue that at mitosis, the changes detected were due to the charge density distribution at the cell surface. Our data demonstrate subtle electrical changes associated with cell motility and for the first time with division at the single-cell level. We speculate that this could open up new avenues for characterizing healthy and pathological cells.
Authors:
Lamya Ghenim; Hirokazu Kaji; Yu Hoshino; Takeshi Ishibashi; Vincent Haguet; Xavier Gidrol; Matsuhiko Nishizawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-08-02
Journal Detail:
Title:  Lab on a chip     Volume:  10     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-09-07     Completed Date:  2010-11-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  2546-50     Citation Subset:  IM    
Affiliation:
CEA, iRTSV, Laboratoire Biopuces, 17 Rue des Martyrs, 38054 Grenoble, France. lamya.ghenim@cea.fr
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MeSH Terms
Descriptor/Qualifier:
Cell Culture Techniques / instrumentation*
Cell Movement / physiology*
Conductometry / instrumentation*
Equipment Design
Equipment Failure Analysis
Hela Cells
Humans
Microfluidic Analytical Techniques / instrumentation*
Mitosis / physiology*
Plethysmography, Impedance / instrumentation*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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