Document Detail


Monitoring the human beta1, beta2, beta3 adrenergic receptors expression and purification in Pichia pastoris using the fluorescence properties of the enhanced green fluorescent protein.
MedLine Citation:
PMID:  18797996     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The three beta adrenergic receptor subtypes, beta1-, beta2- and beta3-, were expressed in the methylotrophic yeast Pichia pastoris. These receptors were N-terminally fused to the enhanced green fluorescent protein (EGFP) and the fluorescent properties of EGFP were used: (1) to select the recombinant strains, (2) to monitor the expression of the fluorescent receptors, and (3) to monitor the purification of the receptors by immobilized metal affinity chromatography. We demonstrate here that Pichia pastoris can be an alternative host to express and purify milligram amounts of human beta adrenergic receptors.
Authors:
Franck Talmont
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-17
Journal Detail:
Title:  Biotechnology letters     Volume:  31     ISSN:  1573-6776     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-11-21     Completed Date:  2008-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  49-55     Citation Subset:  IM    
Affiliation:
Institut de Pharmacologie et de Biologie Structurale (UMR 5089), Centre National de la Recherche Scientifique-Université de Toulouse, 205 route de Narbonne, 31077 Toulouse Cedex 4, France. franck.talmont@ipbs.fr
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MeSH Terms
Descriptor/Qualifier:
Fluorescence
Green Fluorescent Proteins / genetics,  metabolism*
Humans
Immunoblotting
Kinetics
Pichia / metabolism*
Receptors, Adrenergic, beta / genetics,  isolation & purification*,  metabolism*
Receptors, Adrenergic, beta-1 / genetics,  isolation & purification,  metabolism
Receptors, Adrenergic, beta-2 / genetics,  isolation & purification,  metabolism
Receptors, Adrenergic, beta-3 / genetics,  isolation & purification,  metabolism
Silver Nitrate
Solubility
Chemical
Reg. No./Substance:
0/Receptors, Adrenergic, beta; 0/Receptors, Adrenergic, beta-1; 0/Receptors, Adrenergic, beta-2; 0/Receptors, Adrenergic, beta-3; 0/enhanced green fluorescent protein; 147336-22-9/Green Fluorescent Proteins; 7761-88-8/Silver Nitrate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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