Document Detail


Monitoring the growth and drug susceptibility of individual bacteria using asynchronous magnetic bead rotation sensors.
MedLine Citation:
PMID:  21095112     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Continuous growth of individual bacteria has been previously studied by direct observation using optical imaging. However, optical microscopy studies are inherently diffraction limited and limited in the number of individual cells that can be continuously monitored. Here we report on the use of the asynchronous magnetic bead rotation (AMBR) sensor, which is not diffraction limited. The AMBR sensor allows for the measurement of nanoscale growth dynamics of individual bacterial cells, over multiple generations. This torque-based magnetic bead sensor monitors variations in drag caused by the attachment and growth of a single bacterial cell. In this manner, we observed the growth and division of individual Escherichia coli, with 80-nm sensitivity to the cell length. Over the life cycle of a cell, we observed up to a 300% increase in the rotational period of the biosensor due to increased cell volume. In addition, we observed single bacterial cell growth response to antibiotics. This work demonstrates the non-microscopy limited AMBR biosensor for monitoring individual cell growth dynamics, including cell elongation, generation time, lag time, and division, as well as their sensitivity to antibiotics.
Authors:
Paivo Kinnunen; Irene Sinn; Brandon H McNaughton; Duane W Newton; Mark A Burns; Raoul Kopelman
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-10-14
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  26     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-03     Completed Date:  2011-04-14     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  2751-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology*
Cell Survival / drug effects
Equipment Design
Equipment Failure Analysis
Escherichia coli / drug effects*,  growth & development*
Immunomagnetic Separation / instrumentation*
Rotation
Transducers*
Grant Support
ID/Acronym/Agency:
R21 EB009550/EB/NIBIB NIH HHS; R21 EB009550-01A2/EB/NIBIB NIH HHS; R21 EB009550-02/EB/NIBIB NIH HHS; R21EB009550/EB/NIBIB NIH HHS; UL1RR024986/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents
Comments/Corrections

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