| Monitoring fermentation media by ion-chromatography with a double chamber bulk acoustic wave detector. | |
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MedLine Citation:
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PMID: 18967796 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A reliable ion-chromatographic (IC) method with a novel double chamber bulk acoustic wave (DCBAW) detector was developed for monitoring five important inorganic cations (Na(+), K(+), NH(4)(+), Ca(2+), Mg(2+)) in biological culture media. A Shimpack IC-C1 analytical column with 5 mM hydrochloric acid and 2 mM acetonitrile as mobile phase was used. All investigated inorganic cations could be detected and qualified in the range of 0.1-100 mg/l. Results showed that the consumption velocities of Mg(2+) and K(+) are related to the growth of the cells and decrease fastest during the first 2-5 h. Mg became a growth limiting factor at concentration below 0.1 mg/l. The concentrations of the other cations stayed nearly constant during the whole fermentation process. The simple sample preparation, short analytical time and accurate results made it a useful tool for the on-line monitoring, controlling and optimization of the fermentation process. |
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Authors:
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Y T Xie; H S Zeng; S Z Yao; W Z Wei |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Talanta Volume: 50 ISSN: 0039-9140 ISO Abbreviation: Talanta Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2008-10-30 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
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Languages: eng Pagination: 1019-25 Citation Subset: - |
Affiliation:
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Chemistry and Chemical Engineering College, Hunan University, Hunan, Changsha 410082, People's Republic of China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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