| Monitoring epidemic viral respiratory infections using one-step real-Time Triplex RT-PCR targeting influenza A and B viruses and respiratory syncytial virus. | |
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MedLine Citation:
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PMID: 21328385 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Rapid and specific diagnosis of influenza A/B and respiratory syncytial virus (RSV) viruses is needed for optimal management of patients with acute respiratory infections. In this study, a one-step triplex real-time RT-PCR assay was developed for rapid diagnosis of influenza A/B and RSV infections to optimize diagnosis efficiency of acute respiratory infections. Cell-culture supernatants and clinical samples were used to evaluate specificity and sensitivity of the assay. The assay was used routinely during two winter epidemics for testing respiratory specimens from 2,417 patients. The limit of detection in cell-culture supernatant was 1-10 plaque forming units/input (influenza A/B) and 2 × 10(-2) 50% tissue culture infectious dose/input (RSV). In clinical samples, the assay was as sensitive as commercial molecular assays for the detection of each influenza A/B and RSV (Flu-A/B and RSV-A/B r-gene™) individually, and far more sensitive than antigen detection. During the winter 2008-2009, the assay identified 145 RSV, 42 influenza A, and one mixed RSV-influenza A infections among 298 patients. The next winter, the assay was used in two independent hospital laboratory settings. 776 patients were tested in one hospital and 1,343 in the other, resulting in 184 and 501 RSV, 133 and 150 influenza A, and 1 and 11 mixed RSV-influenza A infections, respectively, being detected. This new user-friendly assay allows rapid (within hours), effective molecular diagnosis of single or mixed infections involving influenza A (including seasonal A H1N1 and H3N2, and A(H1N1) 2009), influenza B, and RSV(A/B). The assay is very valuable for managing patients during winter epidemics when influenza and respiratory syncytial viruses co-circulate. J. Med. Virol. 83:695-701, 2011. © 2011 Wiley-Liss, Inc. |
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Authors:
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Solesne Papillard-Marechal; Vincent Enouf; Aurélie Schnuriger; Astrid Vabret; Edouard Macheras; Marie-Anne Rameix-Welti; Bernard Page; François Freymuth; Sylvie van der Werf; Antoine Garbarg-Chenon; Bertrand Chevallier; Jean-Louis Gaillard; Elyanne Gault |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of medical virology Volume: 83 ISSN: 1096-9071 ISO Abbreviation: J. Med. Virol. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-02-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7705876 Medline TA: J Med Virol Country: United States |
Other Details:
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Languages: eng Pagination: 695-701 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Wiley-Liss, Inc. |
Affiliation:
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AP-HP, Ambroise Paré Hospital, Department of Pediatrics, Boulogne-Billancourt, France. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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