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Monitoring the endocytosis of magnetic nanoparticles by cells using permanent micro-flux sources.
MedLine Citation:
PMID:  22773161     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Trapping of cells is essential to perform basic handling operations in cell-based microsystems, such as media exchange, concentration, cell isolation and cell sorting. Cell trapping by magnetophoresis typically requires cell labeling with magnetic nanoparticles. Here we report on endocytotic uptake of 100 nm magnetic nanoparticles by Human Embryonic Kidney 293 cells. The attraction of labeled cells by micro-magnet arrays characterised by very high magnetic field gradients (≤10(6) T/m) was studied as a function of labeling conditions (nanoparticle concentration in the extracellular medium, incubation time). The threshold incubation conditions for effective magnetophoretic trapping were established. This simple technique may be exploited to minimise the quantity of magnetic nanoparticles needed for efficient cell trapping, thus reducing stress or nanoparticle-mediated toxicity. Nanoparticle internalization into cells was confirmed using both confocal and Transmission Electron Microscopy (TEM).
Authors:
O Osman; L F Zanini; M Frénéa-Robin; F Dumas-Bouchiat; N M Dempsey; G Reyne; F Buret; N Haddour
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-7
Journal Detail:
Title:  Biomedical microdevices     Volume:  -     ISSN:  1572-8781     ISO Abbreviation:  -     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-7-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100887374     Medline TA:  Biomed Microdevices     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Ampère Laboratory, CNRS - Université Claude Bernard Lyon 1- Ecole Centrale de Lyon, 69134, Ecully, France, osman.osman@ec-lyon.fr.
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