| Monitoring of cellular responses to hypoxia. | |
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MedLine Citation:
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PMID: 19957135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxygen is essential for survival of aerobic organisms. Sensing changes in the environmental oxygen concentration and appropriate adaptation to such changes are essential for organisms to survive. Hypoxia inducible factor 1 (HIF-1) is the key transcription factor in controlling the expression of oxygen-dependent genes required for this adaptation. HIF-1 is a heterodimer of an oxygen dependent alpha-subunit and constitutive beta-subunit. Abundance and activity of HIF-1 is controlled by post-translational hydroxylation. Microscopic analysis of the assembly and activation process of HIF-1 has become an important tool to better understand HIF-1 regulation. Confocal laser microscopy provides exact images of HIF-1alpha that is translocated into the nucleus under hypoxia and its disappearance upon reoxygenation. To exactly follow the protein-protein interaction during the assembly process of HIF-1, both subunits were labeled by fusing them to fluorescent proteins. Fluorescence resonance energy transfer (FRET) was used to determine the interaction of both subunits in living cells by confocal microscopy. |
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Authors:
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Christoph Wotzlaw; Joachim Fandrey |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 591 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2009-12-03 Completed Date: 2010-02-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 243-55 Citation Subset: IM |
Affiliation:
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Institut f?r Physiologie, Universit?t Duisburg-Essen, Essen, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Cell Hypoxia* Cell Line, Tumor Cell Nucleus / metabolism Cell Survival Fluorescence Resonance Energy Transfer Fluorescent Antibody Technique Hypoxia-Inducible Factor 1, alpha Subunit / chemistry, metabolism Mice Microscopy, Confocal Protein Multimerization Protein Structure, Quaternary |
| Chemical | |
Reg. No./Substance:
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0/Hypoxia-Inducible Factor 1, alpha Subunit |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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