Document Detail


Monitoring cell growth.
MedLine Citation:
PMID:  18432653     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This appendix provides two protocols for monitoring cell growth. Counting cells using a hemacytometer is tedious but it allows one to effectively distinguish live cells from dead cells (using Trypan Blue exclusion). In addition, this procedure is less subject to errors due to cell clumping or heterogeneity of cell size. The use of an electronic cell counter is quicker and easier than counting cells using a hemacytometer. However, an electronic cell counter as currently constructed does not distinguish live from dead cells in a reliable fashion and is subject to error due to the presence of cell clumps. Overall, the electronic cell counter is best reserved for repetitive and rapid counting of fresh peripheral blood cells and should be used with caution when counting cell populations derived from tissues.
Authors:
W Strober
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in immunology / edited by John E. Coligan ... [et al.]     Volume:  Appendix 3     ISSN:  1934-368X     ISO Abbreviation:  Curr Protoc Immunol     Publication Date:  2001 May 
Date Detail:
Created Date:  2008-04-24     Completed Date:  2008-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9101651     Medline TA:  Curr Protoc Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Appendix 3A     Citation Subset:  IM    
Affiliation:
National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Count / methods*
Cell Growth Processes / physiology*
Cytological Techniques / instrumentation,  methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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