Document Detail

Monitoring autophagy in the filamentous fungus Podospora anserina.
MedLine Citation:
PMID:  19185726     Owner:  NLM     Status:  MEDLINE    
Autophagy has been monitored in the filamentous fungus Podospora anserina using electron, light, and fluorescence microscopy. In this organism autophagy can be induced either by starvation or rapamycin treatment or by het gene incompatibility. Incompatible HET products signal a cell death reaction referred to as cell death by incompatibility. In het-R het-V strain bearing the two incompatible het-R and het-V genes, cell death is induced by a simple shift in growth temperature, as incompatibility is thermosensitive. In this strain large autophagosomes are formed as revealed by electron microscopy or using the GFP-PaATG8 marker. This strain constitutes an alternative model to study autophagy. Analysis of the three autophagy mutants, DeltaPaATG1, DeltaPaATG8, and DeltapspA, reveals that autophagy is essential for aerial hyphae and female organ differentiation and involved in spore germination. During the incompatibility reaction, autophagy might protect cells from cell death as suggested by accelerated cell death observed in autophagy mutants.
Bérangère Pinan-Lucarré; Corinne Clavé
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in enzymology     Volume:  451     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2008  
Date Detail:
Created Date:  2009-02-02     Completed Date:  2009-03-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  251-70     Citation Subset:  IM    
Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, Université de Bordeaux 2 et CNRS, Bordeaux, France.
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MeSH Terms
Autophagy / physiology*
Biological Assay / methods*
Fungal Proteins / genetics,  metabolism
Microscopy, Electron / methods
Phagosomes / ultrastructure
Podospora / physiology*,  ultrastructure
Recombinant Fusion Proteins / genetics,  metabolism
Spores, Fungal / metabolism
Reg. No./Substance:
0/Fungal Proteins; 0/Recombinant Fusion Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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