Document Detail


Monitoring of arrays of amino acids in clinical samples using capillary electrophoresis with contactless conductivity detection.
MedLine Citation:
PMID:  22976086     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Capillary electrophoresis (CE) with contactless conductivity detection (C(4)D) is readily applicable to determinations of amino acids in clinical samples. Most of these analyses employ long separation pathways. This chapter describes CE/C(4)D determinations of 28 biogenic amino acids in a short capillary with an effective length of 18 cm. All the test amino acids can be mutually separated in electrolytes of 0.5-10 mol/L acetic acid. The time of analysis does not exceed 6 min; the limits of detection vary from 0.1 to 1.7 μmol/L for all the analytes. The pretreatment of the biological material is very simple, consisting of the removal of proteins by an addition of acetonitrile and subsequent filtration. The procedure has been successfully applied to determinations of the whole amino acid spectra in blood plasma, urine, saliva, cerebrospinal, and amniotic fluid samples.
Authors:
Petr Tůma; Karel Stulík
Related Documents :
15974576 - Synthesis and ocular effects of imidazole nitrolic acids.
12700886 - Effects of resiniferatoxin desensitization of capsaicin-sensitive afferents on detrusor...
23186686 - Succinic acid production from cellobiose by actinobacillus succinogenes.
16960076 - The effects of lactobacillus buchneri 40788 and pediococcus pentosaceus r1094 on the fe...
16288526 - Synthesis of conformationally constrained c-glycosyl alpha- and beta-amino acids and su...
23941506 - Underutilized chokeberry (aronia melanocarpa, arbutifolia, prunifolia) accessions are r...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  919     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-09-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  13-23     Citation Subset:  IM    
Affiliation:
Institute of Biochemistry, Cell and Molecular Biology, Charles University, Prague, Czech Republic, petr.tuma@lf3.cuni.cz.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  An Overview of CE in Clinical Analysis.
Next Document:  The selective determination of sulfates, sulfonates, and phosphates in urine by capillary electropho...