Document Detail


Monitoring the apoptotic process induced by oxidized low-density lipoprotein in Jurkat T-lymphoblast and U937 monocytic human cell lines.
MedLine Citation:
PMID:  15054217     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell death is a major event in the pathophysiology of atherosclerosis. Oxidized low-density lipoprotein (Ox-LDL), which plays a key role in the atherogenesis, has a powerful cytotoxic effect and causes necrosis or apoptosis of different types of cells. In the present work we studied the mechanism of cell death in two model systems: T lymphocytes and monocytes cell line, exposed to Ox-LDL. Ox-LDL, but not native low-density lipoprotein (LDL), was found to be cytotoxic to both cell types in a dose and time dependent manner. Apoptotic cell death was analyzed by evaluating cell size, nucleus DNA content and plasma membrane asymmetry. Early cytoplasmic condensation resulting from cell shrinkage was measured by monitoring fluorescence polarization (FP) of fluorescein labeled cells. The radical scavenger superoxide dismutase (SOD), in a time- and dose-dependent manner, reduced the apoptotic effect of Ox-LDL. Hyperpolarization of fluorescein-labeled cells preceded the appearance of phosphatidylserine (PS) on the plasma membrane. This sensitive parameter for early apoptosis detected different cell death kinetics, as well as varying sensitivity to the inhibitory effect of SOD in monocytes and lymphocytes. Such data suggest that reactive oxygen species generation are involved in Ox-LDL-induced apoptosis and that monocytes are more susceptible to cell death triggered by oxidative stress.
Authors:
Naomi Zurgil; Inna Solodeev; Boris Gilburd; Yana Shafran; Elena Afrimzon; Rami Avtalion; Yehuda Shoenfeld; Mordechai Deutsch
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell biochemistry and biophysics     Volume:  40     ISSN:  1085-9195     ISO Abbreviation:  Cell Biochem. Biophys.     Publication Date:  2004  
Date Detail:
Created Date:  2004-03-31     Completed Date:  2005-11-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9701934     Medline TA:  Cell Biochem Biophys     Country:  United States    
Other Details:
Languages:  eng     Pagination:  97-113     Citation Subset:  IM    
Affiliation:
The Biophysical Interdisciplinary Jerome Schottenstein Center for the Research and Technology of the Cellome, Department of Physics, Bar Ilan University, Ramat Gan, Israel.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects*
Cell Size / drug effects*
Cell Survival / drug effects*
Dose-Response Relationship, Drug
Humans
Jurkat Cells
Lipoproteins, LDL / administration & dosage*
Oxidation-Reduction
U937 Cells
Chemical
Reg. No./Substance:
0/Lipoproteins, LDL; 0/oxidized low density lipoprotein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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