| Monitoring antibody titers to recombinant Core-NS3 fusion polypeptide is useful for evaluating hepatitis C virus infection and responses to interferon-alpha therapy. | |
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MedLine Citation:
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PMID: 10331562 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To evaluate the clinical feasibility of the antibody titer against a chimeric polypeptide (named Core 518), in which a domain of Core and NS3 of hepatitis C virus (HCV) was fused, ELISA was performed in a total of 76 serum samples. Each serum was serially diluted using two-fold dilution method with distilled water into 10 concentrations. They were all positive for second generation anti-HCV assay (HCV EIA II; Abbott Laboratories). Genotyping RT-PCR, quantitative competitive RT-PCR, and RIBA (Lucky Confirm; LG Biotech) were also assayed. Anti-Core 518 antibody was detected in x 12800 or higher dilutions of sera from 35 of 43 chronic hepatitis C (81.4%) and nine of 16 hepatocellular carcinoma sera (56.3%), one of four cirrhosis (25%), 0 of four acute hepatitis C, and one of nine healthy isolated anti-HCV-positive subjects (p=0.0000). The anti-Core 518 antibody titers were well correlated with the presence of HCV RNA in serum (p=0.002). The anti-Core 518 antibody titers decreased significantly in nine of ten responders to IFN-alpha treatment. Monitoring anti-Core 518 titers may be helpful not only for differentiating the status of HCV infection among patients with various type C viral liver diseases, but also for predicting responses to IFN-alpha treatment. |
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Authors:
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Y M Park; B H Byun; J Y Choi; S H Bae; B S Kim; H S So; W S Ryu |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of Korean medical science Volume: 14 ISSN: 1011-8934 ISO Abbreviation: J. Korean Med. Sci. Publication Date: 1999 Apr |
Date Detail:
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Created Date: 1999-07-19 Completed Date: 1999-07-19 Revised Date: 2011-03-16 |
Medline Journal Info:
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Nlm Unique ID: 8703518 Medline TA: J Korean Med Sci Country: KOREA (SOUTH) |
Other Details:
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Languages: eng Pagination: 165-70 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, Kangnam St. Mary's Hospital, Seoul, Korea. ympark@cmc.cuk.ac.kr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Female Genotype Hepacivirus / genetics, immunology* Hepatitis C / blood, diagnosis, drug therapy*, immunology* Hepatitis C Antibodies / blood, immunology* Hepatitis C Antigens / immunology* Humans Immunoblotting Interferon Alfa-2a / therapeutic use* Male Middle Aged RNA, Viral / blood Recombinant Fusion Proteins / immunology Viral Core Proteins / immunology* Viral Nonstructural Proteins / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Hepatitis C Antibodies; 0/Hepatitis C Antigens; 0/NS3 protein, hepatitis C virus; 0/RNA, Viral; 0/Recombinant Fusion Proteins; 0/Viral Core Proteins; 0/Viral Nonstructural Proteins; 0/nucleocapsid protein, Hepatitis C virus; 76543-88-9/Interferon Alfa-2a |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Korean Med Sci Journal ID (pmc): jkms ISSN: 1011-8934 ISSN: 1598-6357 Publisher: Korean Academy of Medical Sciences |
Article Information Download PDF  Print publication date: Month: 4 Year: 1999 Volume: 14 Issue: 2 First Page: 165 Last Page: 170 ID: 3054362 PubMed Id: 10331562 |
| Monitoring antibody titers to recombinant Core-NS3 fusion polypeptide is useful for evaluating hepatitis C virus infection and responses to interferon-alpha therapy. | |
| Y. M. Park | Email: ympark@cmc.cuk.ac.kr |
| B. H. Byun | |
| J. Y. Choi | |
| S. H. Bae | |
| B. S. Kim | |
| H. S. So | |
| W. S. Ryu | |
| Department of Internal Medicine, Kangnam St. Mary's Hospital, Seoul, Korea. |
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