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Monitoring angiogenesis using a human compatible calibration for broadband near-infrared spectroscopy.
MedLine Citation:
PMID:  23314579     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
ABSTRACT. Angiogenesis is a hallmark of many conditions, including cancer, stroke, vascular disease, diabetes, and high-altitude exposure. We have previously shown that one can study angiogenesis in animal models by using total hemoglobin (tHb) as a marker of cerebral blood volume (CBV), measured using broadband near-infrared spectroscopy (bNIRS). However, the method was not suitable for patients as global anoxia was used for the calibration. Here we determine if angiogenesis could be detected using a calibration method that could be applied to patients. CBV, as a marker of angiogenesis, is quantified in a rat cortex before and after hypoxia acclimation. Rats are acclimated at 370-mmHg pressure for three weeks, while rats in the control group are housed under the same conditions, but under normal pressure. CBV increased in each animal in the acclimation group. The mean CBV (%volume/volume) is 3.49%±0.43% (mean±SD) before acclimation for the experimental group, and 4.76%±0.29% after acclimation. The CBV for the control group is 3.28%±0.75%, and 3.09%±0.48% for the two measurements. This demonstrates that angiogenesis can be monitored noninvasively over time using a bNIRS system with a calibration method that is compatible with human use and less stressful for studies using animals.
Authors:
Runze Yang; Qiong Zhang; Ying Wu; Jeff F Dunn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  18     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  16011     Citation Subset:  IM    
Affiliation:
University of Calgary, Department of Radiology, Calgary, Alberta, CanadabUniversity of Calgary, Hotchkiss Brain Institute, Calgary, Alberta, Canada.
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