Document Detail

Monitoring the activity of tyrosinase on a tyramine/dopamine-functionalized surface by force microscopy.
MedLine Citation:
PMID:  17567175     Owner:  NLM     Status:  MEDLINE    
Tyrosinase activity is monitored by pi-donor-acceptor force interactions between a bipyridinium-modified AFM tip and the biocatalytic reaction product generated on a tyramine- (or dopamine-) modified surface. Upon oxidation of the surface to dopaquinone as a result of tyrosinase activity, force interactions are switched "OFF". After reduction of the resulting surface with ascorbic acid, forces are quantitatively reestablished as a result of the formation of the dopamine-functionalized surfaces. The method provides a general approach to design biosensors using force interactions as the readout signal.
Adam B Braunschweig; Roey Elnathan; Itamar Willner
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Publication Detail:
Type:  Journal Article; Review     Date:  2007-06-14
Journal Detail:
Title:  Nano letters     Volume:  7     ISSN:  1530-6984     ISO Abbreviation:  Nano Lett.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-07-11     Completed Date:  2007-08-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088070     Medline TA:  Nano Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2030-6     Citation Subset:  IM    
Institute of Chemistry and the Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
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MeSH Terms
Biosensing Techniques / instrumentation
Dopamine* / chemistry,  metabolism
Microscopy, Atomic Force*
Monophenol Monooxygenase / analysis*,  chemistry,  physiology
Tyramine* / chemistry,  metabolism
Reg. No./Substance:
51-67-2/Tyramine; EC Monooxygenase

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