| Monitoring of WT1 expression in PB and CD34(+) donor chimerism of BM predicts early relapse in AML and MDS patients after hematopoietic cell transplantation with reduced-intensity conditioning. | |
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MedLine Citation:
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PMID: 21135860 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Relapse of malignant disease remains the major complication in patients with acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS) after hematopoietic cell transplantation (HCT) with reduced-intensity conditioning (RIC). In this study, we investigated the predictive value of disease-specific markers (DSMs), donor chimerism (DC) analysis of unsorted (UDC) or CD34(+) sorted cells and Wilms' tumor gene 1 (WT1) expression. Eighty-eight patients with AML or MDS were monitored after allogenic HCT following 2 Gy total-body irradiation with (n=84) or without (n=4) fludarabine 3 × 30 mg/m(2), followed by cyclosporin A and mycophenolate mofetil. DSMs were determined by fluorescence in situ hybridization (FISH) and WT1 expression by real-time polymerase chain reaction. Chimerism analysis was performed on unsorted or CD34(+) sorted cells, by FISH or short tandem repeat polymerase chain reaction. Twenty-one (24%) patients relapsed within 4 months after HCT. UDC, CD34(+) DC and WT1 expression were each significant predictors of relapse with sensitivities ranging from 53 to 79% and specificities of 82-91%. Relapse within 28 days was excluded almost entirely on the basis of WT1 expression combined with CD34(+) DC kinetics. Monitoring of WT1 expression and CD34(+) DC predict relapse of AML and MDS after RIC-HCT. |
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Authors:
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T Lange; M Hubmann; R Burkhardt; G-N Franke; M Cross; M Scholz; S Leiblein; H K Al-Ali; J Edelmann; J Thiery; D Niederwieser |
Publication Detail:
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Type: Journal Article Date: 2010-12-07 |
Journal Detail:
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Title: Leukemia Volume: 25 ISSN: 1476-5551 ISO Abbreviation: Leukemia Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-03-09 Completed Date: 2011-05-13 Revised Date: 2013-03-04 |
Medline Journal Info:
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Nlm Unique ID: 8704895 Medline TA: Leukemia Country: England |
Other Details:
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Languages: eng Pagination: 498-505 Citation Subset: IM |
Affiliation:
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Department of Hematology, Oncology and Hemostaseology, University of Leipzig, Leipzig, Germany. langet@medizin.uni-leipzig.de |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Antigens, CD34 / analysis* Blood Donors Disease-Free Survival Female Genes, Wilms Tumor* Hematopoietic Stem Cell Transplantation* Humans In Situ Hybridization, Fluorescence Leukemia, Myeloid, Acute / genetics*, mortality, therapy Male Middle Aged Myelodysplastic Syndromes / genetics*, mortality, therapy Recurrence Transplantation Chimera Transplantation Conditioning* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD34 |
| Comments/Corrections | |
Comment In:
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Expert Rev Mol Diagn. 2011 May;11(4):361-6
[PMID:
21545254
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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