Document Detail


Monitoring Ultraviolet (UV) Radiation Inactivation of Cronobacter sakazakii in Dry Infant Formula Using Fourier Transform Infrared Spectroscopy.
MedLine Citation:
PMID:  22182052     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
  Cronobacter sakazakii is an opportunistic pathogen associated with dry infant formula presenting a high risk to low birth weight neonates. The inactivation of C. sakazakii in dry infant formula by ultraviolet (UV) radiation alone and combined with hot water treatment at temperatures of 55, 60, and 65 °C were applied in this study. UV radiation with doses in a range from 12.1 ± 0.30 kJ/m(2) to 72.8 ± 1.83 kJ/m(2) at room temperature demonstrated significant inactivation of C. sakazakii in dry infant formula (P < 0.05). UV radiation combining 60 °C hot water treatment increased inactivation of C. sakazakii cells significantly (P < 0.05) in reconstituted infant formula. Significant effects of UV radiation on C. sakazakii inactivation kinetics (D value) were not observed in infant formula reconstituted in 55 and 65 °C water (P > 0.05). The inactivation mechanism was investigated using vibrational spectroscopy. Infrared spectroscopy detected significant stretching mode changes of macromolecules on the basis of spectral features, such as DNA, proteins, and lipids. Minor changes on cell membrane composition of C. sakazakii under UV radiation could be accurately and correctly monitored by infrared spectroscopy coupled with 2nd derivative transformation and principal component analysis.
Authors:
Qian Liu; Xiaonan Lu; Barry G Swanson; Barbara A Rasco; Dong-Hyun Kang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-12-19
Journal Detail:
Title:  Journal of food science     Volume:  -     ISSN:  1750-3841     ISO Abbreviation:  -     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-12-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0014052     Medline TA:  J Food Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Institute of Food Technologists®
Affiliation:
Authors Liu, Lu, Swanson, and Rasco are with School of Food Science, Washington State Univ., Pullman, WA 99163, U.S.A. Author Lu is also with Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin Univ. of Science and Technology, Tianjin 300457, China. Author Kang is with Dept. of Food and Animal Biotechnology, Dept. of Agricultural Biotechnology, Center for Agricultural Biomaterials, and Research Inst. for Agriculture and Life Sciences, Seoul Natl. Univ., Seoul 151-921, Republic of Korea. Direct inquiries to author Kang (E-mail: kang7820@snu.ac.kr).
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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