Document Detail


Monitoring of Saccharomyces cerevisiae cell proliferation on thiol-modified planar gold microelectrodes using impedance spectroscopy.
MedLine Citation:
PMID:  18630975     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An impedance spectroscopic study of the interaction between thiol-modified Au electrodes and Saccharomyces cerevisiae of strain EBY44 revealed that the cells formed an integral part of the interface, modulating the capacitive properties until a complete monolayer was obtained, whereas the charge transfer resistance ( R ct) to the redox process of [Fe(CN)6] 3-/4- showed a linear relationship to the number of cells even beyond the monolayer coverage. R ct showed strong pH dependence upon increasing the pH of the utilized buffer to 7.2. Upon addition of S. cerevisiae cells at pH 7.2, the obtained value of R ct showed over 560% increase with respect to the value obtained on the same thiol-modified electrode without cells. It was demonstrated that real-time monitoring of S. cerevisiae proliferation, with frequency-normalized imaginary admittance (real capacitance) as the indicator, was possible using a miniaturized culture system, ECIS Cultureware, with integrated planar cysteamine-modified Au microelectrodes. A monolayer coverage was reached after 20-28 h of cultivation, observed as an approximately 15% decrease in the real capacitance of the system.
Authors:
Arto R Heiskanen; Christer F Spégel; Natalie Kostesha; Tautgirdas Ruzgas; Jenny Emnéus
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-17
Journal Detail:
Title:  Langmuir : the ACS journal of surfaces and colloids     Volume:  24     ISSN:  0743-7463     ISO Abbreviation:  Langmuir     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-14     Completed Date:  2008-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882736     Medline TA:  Langmuir     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9066-73     Citation Subset:  IM    
Affiliation:
Department of Analytical Chemistry, Lund University, PO Box 124, Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Cell Proliferation
Electric Impedance
Gold / chemistry*
Microelectrodes
Saccharomyces cerevisiae / cytology*
Spectrum Analysis
Sulfhydryl Compounds / chemistry*
Chemical
Reg. No./Substance:
0/Sulfhydryl Compounds; 7440-57-5/Gold

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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