Document Detail


Monitoring Rhodotorula glutinis CCMI 145 physiological response and oil production growing on xylose and glucose using multi-parameter flow cytometry.
MedLine Citation:
PMID:  21030251     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Flow cytometry was used to monitor the lipid content, viability and intrinsic light scatter properties of Rhodotorula glutinis CCMI 145 cells growing on batch cultures using xylose and glucose as carbon sources. The highest lipid content was observed for cells grown on glucose, at the end of the exponential phase (17.8% w/w). The proportion of cells stained with PI attaining 77% at the end of the glucose growth. Cells growing on xylose produced a maximum lipid content of 10.6% (w/w), at the stationary phase. An increase in the proportion of cells stained with PI was observed, reaching 29% at the end of xylose growth. Changes in the side and forward light scatter detected during the yeast batch cultures supported that R. glutinis cells grown on glucose experienced harsher conditions, resulting in a high level of cytoplasmic membrane damage, which did not occur when R. glutinis cells grew on xylose.
Authors:
Teresa Lopes da Silva; Daniela Feijão; José Carlos Roseiro; Alberto Reis
Publication Detail:
Type:  Journal Article     Date:  2010-10-08
Journal Detail:
Title:  Bioresource technology     Volume:  102     ISSN:  1873-2976     ISO Abbreviation:  Bioresour. Technol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9889523     Medline TA:  Bioresour Technol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2998-3006     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Ltd. All rights reserved.
Affiliation:
Laboratório Nacional de Energia e Geologia (LNEG), Unidade de Bioenergia, Estrada do Paço do Lumiar, Lisboa, Portugal. teresa.lopesilva@lneg.pt
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