Document Detail


Monitoring N-nitrosamino acids excreted in the urine and feces of rats as an index for endogenous nitrosation.
MedLine Citation:
PMID:  6175434     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and sensitive method for the quantitative estimation of endogenous N-nitrosation in rats has been developed. This approach is based on the findings that N-nitrosamino acids (e.g., nitrosoproline (NPRO), nitrosohydroxyproline (NHPRO) and nitrososarcosine (NSAR)) when administered orally to rats, are excreted unchanged almost quantitatively (88-96% of the dose) in the urine and feces. After sequential administration of a nitrosatable amino acid and sodium nitrite the nitrosamino acid excreted in the urine and feces was analyzed. The amount of NPRO excreted in the urine of rats was proportional to the dose of proline and to the square of the nitrite dose. Co-administration of ascorbic acid and alpha-tocopherol together with the precursors decreased the urinary NPRO whereas thiocyanate increased the yield. After feeding an amino acid precursor and nitrite, the yield of nitrosamino acids formed in vivo and excreted in the urine increased in the order: NPRO less than NSAR less than NHPRO. The same order was seen when the nitrosation rates of the amino acids in vitro were compared. Thus N-nitrosation in vivo in rats occurs via a similar mechanism as observed in vitro. Monitoring of N-nitrosamino acids excreted in the urine and feces thus appears to provide a valuable index for endogenous N-nitrosation.
Authors:
H Ohshima; J C Bereziat; H Bartsch
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Carcinogenesis     Volume:  3     ISSN:  0143-3334     ISO Abbreviation:  Carcinogenesis     Publication Date:  1982  
Date Detail:
Created Date:  1982-06-24     Completed Date:  1982-06-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8008055     Medline TA:  Carcinogenesis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  115-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Animals
Ascorbic Acid / pharmacology
Feces / analysis
Hydroxyproline / metabolism
Male
Nitrosamines / metabolism
Nitroso Compounds / metabolism*
Proline / metabolism
Rats
Rats, Inbred Strains
Sarcosine / metabolism
Sodium Nitrite / metabolism
Vitamin E / pharmacology
Grant Support
ID/Acronym/Agency:
N01 CP-55715/CP/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Nitrosamines; 0/Nitroso Compounds; 107-97-1/Sarcosine; 1406-18-4/Vitamin E; 147-85-3/Proline; 50-81-7/Ascorbic Acid; 51-35-4/Hydroxyproline; 7632-00-0/Sodium Nitrite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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