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Monitoring Glioma Growth and Tumor Necrosis with the U-SPECT-II/CT Scanner by Targeting Integrin αvβ3.
MedLine Citation:
PMID:  23348790     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
AbstractThe purpose of this study was to validate 99mTc-3P-RGD2 single-photon emission computed tomography/computed tomography (SPECT/CT) as an imaging tool to monitor αvβ3 expression and tumor necrosis. The animal model was established by subcutaneous injection of 5 × 106 U87MG cells into the shoulder flank of each mouse. Imaging was performed using the U-SPECT-II/CT scanner (Milabs, Utrecht, the Netherlands). Tumor volumes were determined, and the tumor uptake of 99mTc-3P-RGD2 was calculated on the basis of SPECT/CT and compared to that from biodistribution. Immunohistochemistry was performed to determine CD31 and αvβ3 expression levels. We found that the tumor detection limit was ≈ 0.5 mm3 by 99mTc-3P-RGD2 SPECT/CT. The tumor uptake of 99mTc-3P-RGD2 from SPECT/CT was almost identical to that from biodistribution. The αvβ3 was expressed mainly on blood vessels for the tumors of 0.2 to 0.5 cm3. In larger tumors, tumor αvβ3 expression increased due to more contribution from glioma cells. When tumors were > 0.5 cm3, the %ID/cm3 uptake of 99mTc-3P-RGD2 decreased because of necrosis. The overall relationship between the tumor size and %ID of 99mTc-3P-RGD2 was modeled as a quadratic polynomial fitting curve, with R2 being > .95. 99mTc-3P-RGD2 SPECT/CT is excellent for monitoring αvβ3 expression and tumor necrosis during tumor growth and may become a screening tool for patient selection before anti-αvβ3 therapy.
Authors:
Guoqiang Shao; Yang Zhou; Feng Wang; Shuang Liu
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular imaging     Volume:  12     ISSN:  1536-0121     ISO Abbreviation:  Mol Imaging     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-01-25     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120118     Medline TA:  Mol Imaging     Country:  United States    
Other Details:
Languages:  eng     Pagination:  39-48     Citation Subset:  IM    
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