Document Detail


Monitoring of Escherichia coli O157:H7 in food samples using lectin based surface plasmon resonance biosensor.
MedLine Citation:
PMID:  23194527     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A novel surface plasmon resonance (SPR) biosensor using lectin as bioreceptor was developed for the rapid detection of Escherichia coli (E. coli) O157:H7. The selective interaction of lectins with carbohydrate components from bacterial cells surface was used as the recognition principle for the detection. Five types of lectins from Triticum vulgaris, Canavailia ensiformis, Ulex europaeus, Arachis hypogaea, and Maackia amurensis, were employed to evaluate the selectivity of the approach for binding E. coli O157:H7 effectively. A detection limit of 3×10(3)cfumL(-1) was obtained for determination of E. coli O157:H7 when used the lectin from T. vulgaris as the binding molecule. Furthermore, the proposed biosensor was used to detect E. coli O157:H7 in real food samples. Results showed that the lectin based SPR biosensor was sensitive, reliable and effective for detection of E. coli O157:H7, which hold a great promise in food safety analysis.
Authors:
Yixian Wang; Zunzhong Ye; Chengyan Si; Yibin Ying
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Publication Detail:
Type:  Journal Article     Date:  2012-09-28
Journal Detail:
Title:  Food chemistry     Volume:  136     ISSN:  0308-8146     ISO Abbreviation:  Food Chem     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2012-11-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7702639     Medline TA:  Food Chem     Country:  England    
Other Details:
Languages:  eng     Pagination:  1303-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, PR China.
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