Document Detail

Monitoring of Bt11 and Bt176 genetically modified maize in food sold commercially in Brazil from 2005 to 2007.
MedLine Citation:
PMID:  20549814     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The first genetically modified (GM) maize lines were approved for trading in Brazil after December 2007 and they were T25, MON810, Bt11, NK603 and GA21. The polymerase chain reaction (PCR) method was employed to monitor the presence of Bt11 and nested PCR was used to detect the presence of Bt176 in 81 maize-derived products (maize flour, corn meal, maize flour flakes and polenta) that were sold in Brazilian market from 2005 to 2007, before the release of GM maize in Brazil. RESULTS: The PCR detection limit for Bt11 was 10 g kg(-1) and for nested PCR of Bt176 it was 1 g kg(-1). All Brazilian samples analyzed showed no positive signal for these GM maize events. CONCLUSION: Bt11 and Bt176 GM maize lines were not detected by specific PCR in 81 maize-derived food samples sold in Brazil from 2005 to 2007, before the commercial release of GM maize in Brazil. These Brazilian food industries were in compliance with the rules stipulated by the current legislation with respect to consumer requirements about GMO labeling.
Andréia Z Dinon; Kenia T Bosco; Ana Carolina M Arisi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the science of food and agriculture     Volume:  90     ISSN:  1097-0010     ISO Abbreviation:  J. Sci. Food Agric.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-10-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376334     Medline TA:  J Sci Food Agric     Country:  England    
Other Details:
Languages:  eng     Pagination:  1566-9     Citation Subset:  IM    
Departamento de Ciência e Tecnologia de Alimentos, Centro de Ciências Agrárias, Universidade Federal de Santa Catarina, 88034-001 Florianópolis, Santa Catarina, Brazil.
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MeSH Terms
Acetyltransferases / genetics*
Bacterial Proteins / genetics*
DNA, Plant / isolation & purification,  metabolism
Electrophoresis, Agar Gel
Endotoxins / genetics*
Flour / analysis,  standards
Food Inspection* / methods
Food Labeling / legislation & jurisprudence
Hemolysin Proteins / genetics*
Limit of Detection
Plants, Genetically Modified*
Polymerase Chain Reaction / methods
Seeds / genetics*
Zea mays / genetics*
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Plant; 0/Endotoxins; 0/Hemolysin Proteins; 0/insecticidal crystal protein, Bacillus Thuringiensis; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/phosphinothricin N-acetyltransferase

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