| Momordica charantia Extract Induces Apoptosis in Human Cancer Cells through Caspase- and Mitochondria-Dependent Pathways. | |
| | |
MedLine Citation:
|
PMID: 23091557 Owner: NLM Status: PubMed-not-MEDLINE |
Abstract/OtherAbstract:
|
Plants are an invaluable source of potential new anti-cancer drugs. Momordica charantia is one of these plants with both edible and medical value and reported to exhibit anticancer activity. To explore the potential effectiveness of Momordica charantia, methanol extract of Momordica charantia (MCME) was used to evaluate the cytotoxic activity on four human cancer cell lines, Hone-1 nasopharyngeal carcinoma cells, AGS gastric adenocarcinoma cells, HCT-116 colorectal carcinoma cells, and CL1-0 lung adenocarcinoma cells, in this study. MCME showed cytotoxic activity towards all cancer cells tested, with the approximate IC(50) ranging from 0.25 to 0.35 mg/mL at 24 h. MCME induced cell death was found to be time-dependent in these cells. Apoptosis was demonstrated by DAPI staining and DNA fragmentation analysis using agarose gel electrophoresis. MCME activated caspase-3 and enhanced the cleavage of downstream DFF45 and PARP, subsequently leading to DNA fragmentation and nuclear condensation. The apoptogenic protein, Bax, was increased, whereas Bcl-2 was decreased after treating for 24 h in all cancer cells, indicating the involvement of mitochondrial pathway in MCME-induced cell death. These findings indicate that MCME has cytotoxic effects on human cancer cells and exhibits promising anti-cancer activity by triggering apoptosis through the regulation of caspases and mitochondria. |
| | |
Authors:
|
Chia-Jung Li; Shih-Fang Tsang; Chun-Hao Tsai; Hsin-Yi Tsai; Jong-Ho Chyuan; Hsue-Yin Hsu |
Related Documents
:
|
23320197 - Studies on the cytotoxic activities of punica granatum l. var. spinosa (apple punice) e... 23554447 - Cisplatin resistance associated with parp hyperactivation. 22975317 - Orienting the direction of egfr activation. 23549787 - Netrin-1 induced activation of notch signaling mediates glioblastoma cell invasion. 12937987 - Measurement of rapid changes in cell volume by forward light scattering. 16729237 - Redistribution of gfap and alphab-crystallin after thermal stress in c6 glioma cell line. |
Publication Detail:
|
Type: Journal Article Date: 2012-10-04 |
Journal Detail:
|
Title: Evidence-based complementary and alternative medicine : eCAM Volume: 2012 ISSN: 1741-4288 ISO Abbreviation: Evid Based Complement Alternat Med Publication Date: 2012 |
Date Detail:
|
Created Date: 2012-10-23 Completed Date: 2012-10-24 Revised Date: 2013-04-08 |
Medline Journal Info:
|
Nlm Unique ID: 101215021 Medline TA: Evid Based Complement Alternat Med Country: United States |
Other Details:
|
Languages: eng Pagination: 261971 Citation Subset: - |
Affiliation:
|
Institute of Medical Sciences, Tzu Chi University, Hualien 970, Taiwan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The Phenotype of Circulating Follicular-Helper T Cells in Patients with Rheumatoid Arthritis Defines...
Next Document: Are Primo Vessels (PVs) on the Surface of Gastrointestine Involved in Regulation of Gastric Motility...