| Molokhia (Corchorus olitorius L.) extract suppresses transformation of the aryl hydrocarbon receptor induced by dioxins. | |
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MedLine Citation:
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PMID: 16115717 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Dioxins enter the body mainly through diet and cause the various toxicological effects by binding to the cytosolic aryl hydrocarbon receptor (AhR) followed by its transformation. In recent reports, it has been shown that certain natural compounds suppress AhR transformation in vitro. In this study, we demonstrated that ethanolic extract from molokhia, known as Egyptian spinach, showed the strongest suppressive effect on AhR transformation induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in cell-free system using rat hepatic cytosol among 41 kinds of extracts from vegetables and fruits. The molokhia extract also suppressed TCDD-induced AhR transformation in mouse hepatoma Hepa-1c1c7 cells and in intestinal permeability system constructed with human colon adenocarcinoma Caco-2 cells and human hepatoma HepG2 cells. Moreover, oral administration of the molokhia extract (100mg/kg body weight) decreased 3-methylcholanthrene-induced AhR transformation to the control level by inhibiting translocation of the AhR from cytosol into the nucleus in the liver of rats. The molokhia extract-administered rat liver showed a tolerance to TCDD-induced AhR transformation by ex vivo experiment. These results indicate that molokhia is an attractive food for isolation and identification of a natural antagonist for the AhR. |
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Authors:
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Shin Nishiumi; Yoshiyuki Yabushita; Itsuko Fukuda; Rie Mukai; Ken-Ichi Yoshida; Hitoshi Ashida |
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Publication Detail:
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Type: In Vitro; Journal Article Date: 2005-08-22 |
Journal Detail:
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Title: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association Volume: 44 ISSN: 0278-6915 ISO Abbreviation: Food Chem. Toxicol. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2005-12-12 Completed Date: 2006-03-15 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8207483 Medline TA: Food Chem Toxicol Country: England |
Other Details:
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Languages: eng Pagination: 250-60 Citation Subset: IM |
Affiliation:
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Department of Biofunctional Chemistry, Faculty of Agriculture, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cell Line, Tumor Cell Lineage / drug effects Cell Nucleus / drug effects, metabolism Cell-Free System Corchorus / chemistry* Cytochrome P-450 CYP1A1 / metabolism Cytosol / drug effects, metabolism Dioxins / antagonists & inhibitors*, toxicity* Enzyme-Linked Immunosorbent Assay Ethanol Humans Male Mice Permeability Plant Extracts / pharmacology Rats Rats, Sprague-Dawley Receptors, Aryl Hydrocarbon / drug effects, metabolism* Solvents Teratogens / pharmacology Tetrachlorodibenzodioxin / antagonists & inhibitors, pharmacology Transformation, Genetic / drug effects* |
| Chemical | |
Reg. No./Substance:
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0/Dioxins; 0/Plant Extracts; 0/Receptors, Aryl Hydrocarbon; 0/Solvents; 0/Teratogens; 1746-01-6/Tetrachlorodibenzodioxin; 64-17-5/Ethanol; EC 1.14.14.1/Cytochrome P-450 CYP1A1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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