Document Detail


Molecularly imprinted polymer solid-phase extraction for detection of zearalenone in cereal sample extracts.
MedLine Citation:
PMID:  20579483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this work was to develop a method for the clean-up and preconcentration of zearalenone from corn and wheat samples employing molecularly imprinted polymer (MIP) as selective sorbent for solid-phase extraction (SPE). Cereal samples were extracted with acetonitrile/water (75:25, v/v) and the extract was diluted with water and applied to an AFFINIMIP ZON MIP-SPE column. The column was then washed to eliminate the interferences and zearalenone was eluted with methanol and quantified using HPLC with fluorescence detection (lambda(exc)=275/lambda(em)=450 nm). The precision and accuracy of the method were satisfactory for both cereals at the different fortification levels tested and it gave recoveries between 82 and 87% (RSDr 2.5-6.2%, n=3) and 86 and 90% (RSDr 0.9-6.8%, n=3) for wheat and maize, respectively. MIP-SPE column capacity was determined to be not less than 6.6 microg of zearalenone and to be at least four times higher than that of immunoaffinity column (IAC). The application of AFFINIMIP ZON molecularly imprinted polymer as a selective sorbent material for detection of zearalenone fulfilled the method performance criteria required by the Commission Regulation (EC) No. 401/2006, demonstrating the suitability of the technique for the control of zearalenone in cereal samples.
Authors:
Paolo Lucci; Delphine Derrien; Florent Alix; Céline Pérollier; Sami Bayoudh
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-12
Journal Detail:
Title:  Analytica chimica acta     Volume:  672     ISSN:  1873-4324     ISO Abbreviation:  Anal. Chim. Acta     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-09-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370534     Medline TA:  Anal Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  15-9     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier B.V. All rights reserved.
Affiliation:
POLYINTELL, Pharma Parc II, voie de l'innovation, chaussée du vexin, 27100 Val de Reuil, France.
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MeSH Terms
Descriptor/Qualifier:
Molecular Imprinting*
Polymers / chemistry*
Solid Phase Extraction / methods*
Triticum / chemistry*
Zea mays / chemistry*
Zearalenone / analysis*
Chemical
Reg. No./Substance:
0/Polymers; 17924-92-4/Zearalenone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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