| Molecularly imprinted hydrogel displaying reduced non-specific binding and improved protein recognition. | |
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MedLine Citation:
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PMID: 20397214 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel approach for enhancing protein recognition in molecularly imprinted hydrogel (MIH) is presented. This approach was developed based on the hypothesis that the number of specific binding sites created in the previously described MIH is very small, thus attempts to enhance the capacity result in most cases in additional non-specific binding and loss of selectivity. Thus, blocking the non-specific binding sites could lead to higher capacities and better selectivity. To test this hypothesis, MIH interpenetrating networks designed to block non-specific binding sites were synthesized using two separate stages of polymerization. Re-binding of the template protein (lysozyme) and a competitor protein (cytochrome C) was measured, and the results were compared with the similar experiment performed using a control non-imprinted hydrogel and a "conventional" MIH. The imprinting efficacy of the MIH interpenetrating network was found to be much higher than that of the controls. Furthermore, competitive adsorption assays have demonstrated the superiority of the new formulation. |
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Authors:
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Ortal Yom Tov; Shlomit Luvitch; Havazelet Bianco-Peled |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of separation science Volume: 33 ISSN: 1615-9314 ISO Abbreviation: J Sep Sci Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-31 Completed Date: 2010-09-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101088554 Medline TA: J Sep Sci Country: Germany |
Other Details:
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Languages: eng Pagination: 1673-81 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering and The Russell Berrie Nanotechnology Institute, Technion-Israel Institute of Technology, Haifa, Israel. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adsorption Animals Chemistry Techniques, Analytical Chickens Cytochromes c / chemistry Horses Hydrogels* Hydrogen-Ion Concentration Muramidase / chemistry Proteins / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Hydrogels; 0/Proteins; 9007-43-6/Cytochromes c; EC 3.2.1.17/Muramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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