Document Detail

Molecular weight determination of methyl esters of mycolic acids using thermospray mass spectrometry.
MedLine Citation:
PMID:  1486659     Owner:  NLM     Status:  MEDLINE    
Methyl esters of normal fatty acids, corynomycolate and corynomycolenate were used as model compounds for thermospray mass spectrometric procedures for molecular weight determination of the related nocardial mycolic acids. By using ammonium acetate at the positive ion generator, in both cases, a family of ions was produced. The following members were found and corresponded to the adducts: (1) M + H; M + NH4 and M + H + NH4 for methyl esters of normal fatty acids, whereas M + H, M + 2H and M + H + NH4 were the adducts most frequently observed with methyl corynomycolates. The methyl esters of C40-C48 mycolic acids from Rhodococcus rhodochrous exhibited prominent peaks corresponding to adducts M + H + NH4 whereas those corresponding to M + 2H showed slightly lower intensities. The structure M + H had no significant representatives with this subclass of mycolic acids. A similar pattern was observed with methyl esters of C50-C54 mycolic acids from Nocardia asteroides GUH-2. Ion peaks C50-C54 representing adducts M + 2H and M + H + NH4 prevailed in the mass spectrum. In this case, the intensities of peaks corresponding to M + 2H were slightly higher than those of the M + H + NH4. Essentially three main species of nocardomycolic acids were detected: (1) monounsaturated C50:1, C52:1 and C54:1; (2) diunsaturated C50:2, C52:2 and C54:2 and (3) triunsaturated C52:3 and C54:3 mycolic acids. The most abundant mycolic acid was C52:2 followed in decreasing abundance by C52:1, C54:2, C50:2, C52:3 and C54:3 mycolic acids.
T Ioneda; B L Beaman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chemistry and physics of lipids     Volume:  63     ISSN:  0009-3084     ISO Abbreviation:  Chem. Phys. Lipids     Publication Date:  1992 Nov 
Date Detail:
Created Date:  1993-02-19     Completed Date:  1993-02-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0067206     Medline TA:  Chem Phys Lipids     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  41-6     Citation Subset:  IM    
Instituto de Química, University of São Paulo, Brasil.
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MeSH Terms
Corynebacterium diphtheriae / chemistry
Mass Spectrometry / methods*
Molecular Structure
Molecular Weight
Mycolic Acids / chemistry*
Nocardia asteroides / chemistry
Rhodococcus / chemistry
Grant Support
Reg. No./Substance:
0/Mycolic Acids

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