| Molecular weight determination of methyl esters of mycolic acids using thermospray mass spectrometry. | |
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MedLine Citation:
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PMID: 1486659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Methyl esters of normal fatty acids, corynomycolate and corynomycolenate were used as model compounds for thermospray mass spectrometric procedures for molecular weight determination of the related nocardial mycolic acids. By using ammonium acetate at the positive ion generator, in both cases, a family of ions was produced. The following members were found and corresponded to the adducts: (1) M + H; M + NH4 and M + H + NH4 for methyl esters of normal fatty acids, whereas M + H, M + 2H and M + H + NH4 were the adducts most frequently observed with methyl corynomycolates. The methyl esters of C40-C48 mycolic acids from Rhodococcus rhodochrous exhibited prominent peaks corresponding to adducts M + H + NH4 whereas those corresponding to M + 2H showed slightly lower intensities. The structure M + H had no significant representatives with this subclass of mycolic acids. A similar pattern was observed with methyl esters of C50-C54 mycolic acids from Nocardia asteroides GUH-2. Ion peaks C50-C54 representing adducts M + 2H and M + H + NH4 prevailed in the mass spectrum. In this case, the intensities of peaks corresponding to M + 2H were slightly higher than those of the M + H + NH4. Essentially three main species of nocardomycolic acids were detected: (1) monounsaturated C50:1, C52:1 and C54:1; (2) diunsaturated C50:2, C52:2 and C54:2 and (3) triunsaturated C52:3 and C54:3 mycolic acids. The most abundant mycolic acid was C52:2 followed in decreasing abundance by C52:1, C54:2, C50:2, C52:3 and C54:3 mycolic acids. |
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Authors:
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T Ioneda; B L Beaman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Chemistry and physics of lipids Volume: 63 ISSN: 0009-3084 ISO Abbreviation: Chem. Phys. Lipids Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1993-02-19 Completed Date: 1993-02-19 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0067206 Medline TA: Chem Phys Lipids Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 41-6 Citation Subset: IM |
Affiliation:
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Instituto de Química, University of São Paulo, Brasil. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Corynebacterium diphtheriae
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chemistry Esterification Mass Spectrometry / methods* Molecular Structure Molecular Weight Mycolic Acids / chemistry* Nocardia asteroides / chemistry Rhodococcus / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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R01-AI-20 900/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Mycolic Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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