Document Detail

Molecular weight determination of megadalton DNA electrospray ions using charge detection time-of-flight mass spectrometry.
MedLine Citation:
PMID:  8652877     Owner:  NLM     Status:  MEDLINE    
We present results obtained with a novel mass spectrometer capable of determining the mass of multiply charged electrospray ions generated from samples of macromolecules in the megadalton (MDa) size range. The instrument utilizes a sensitive amplifier which can detect the charge on a single ion as it passes through a tube detector. A velocity measurement of an ion with known electrostatic energy provides the ion's mass-to-charge ratio. Simultaneous detection of the ion charge permits a mass assignment to be made for each ion. Electrospray ions of DNA and polymer molecules with masses greater than 1 x 10(6) Da and charge numbers (z) in excess of 425 e(-) are readily detected in this mass spectrometer. The weights of small particles were also measured. The on-axis single-ion detection configuration provides a duty cycle of nearly 100% and extends the practical application of electrospray mass spectrometry to the analysis of very large molecules with relatively inexpensive instrumentation.
S D Fuerstenau; W H Benner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Rapid communications in mass spectrometry : RCM     Volume:  9     ISSN:  0951-4198     ISO Abbreviation:  Rapid Commun. Mass Spectrom.     Publication Date:  1995  
Date Detail:
Created Date:  1996-07-31     Completed Date:  1996-07-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8802365     Medline TA:  Rapid Commun Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1528-38     Citation Subset:  IM    
Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
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MeSH Terms
DNA / analysis*
Mass Spectrometry / methods*
Molecular Weight
Plasmids / analysis*
Reg. No./Substance:

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