Document Detail

Molecular variability in Brycon cf. pesu Müller and Troschel, 1845 (Characiformes: Characidae) from the Araguaia-Tocantins Basin.
MedLine Citation:
PMID:  18273825     Owner:  NLM     Status:  MEDLINE    
Brycon pesu is a small-sized fish distributed throughout the Amazon and Orinoco Basins and other coastal basins of northeastern South America. Brycon cf. pesu specimens from the Araguaia-Tocantins Basin are currently separated into two morphotypes, Brycon sp1 and Brycon sp2, owing to different coloration of their anal fin. Brycon sp2 has a reddish margin stripe on the anal fin which morphologically distinguishes it from Brycon sp1. In the present research, nuclear and mitochondrial markers were used to test the hypothesis that the Brycon sp1 and Brycon sp2 morphotypes are distinct species. Specimens from the two morphotypes were collected from the Lajeado Hydroelectric Plant and the Palmas River in the Araguaia-Tocantins Basin. Thirty-five loci obtained by the amplification of five inter-simple sequence repeat primers were analyzed but no species-specific bands were detected. Electrophoretic profiles obtained from 5S rDNA non-transcribed spacer amplification failed to show any differentiation in morphotypes. These results were corroborated by nucleotide sequence analysis of the mtDNA control region, in which 24 polymorphic nucleotide sites, representing a polymorphism rate of only 5%, were detected. The low rates of polymorphism detected by inter-simple sequence repeat, non-transcribed spacer and mtDNA D-loop markers strongly reject the hypothesis that the two morphotypes Brycon sp1 and Brycon sp2 represent distinct species within Brycon cf. pesu. Further studies are needed to obtain conclusive data on the notion that the coloration of the anal fin is an intraspecific polymorphism, possibly related to environmental factors.
R S Panarari-Antunes; A J Prioli; S M A P Prioli; H F Júlio; C S Agostinho; L M Prioli
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-01
Journal Detail:
Title:  Genetics and molecular research : GMR     Volume:  7     ISSN:  1676-5680     ISO Abbreviation:  Genet. Mol. Res.     Publication Date:  2008  
Date Detail:
Created Date:  2008-02-14     Completed Date:  2008-03-18     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  101169387     Medline TA:  Genet Mol Res     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  95-106     Citation Subset:  IM    
Programa de Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, Maringá, PR, Brasil .
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MeSH Terms
Base Sequence
DNA / genetics,  isolation & purification
DNA, Intergenic / genetics*
DNA, Mitochondrial / genetics*
Fishes / classification,  genetics*
Genetic Markers
Genetic Variation*
Microsatellite Repeats
Nucleic Acid Conformation
Polymerase Chain Reaction
Polymorphism, Genetic
RNA, Ribosomal, 5S / genetics*
Sequence Analysis, DNA
Species Specificity
Reg. No./Substance:
0/DNA, Intergenic; 0/DNA, Mitochondrial; 0/Genetic Markers; 0/RNA, Ribosomal, 5S; 9007-49-2/DNA

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