Document Detail

Molecular systematics of the neotropical genus Psiguria (Cucurbitaceae): Implications for phylogeny and species identification.
MedLine Citation:
PMID:  21622376     Owner:  NLM     Status:  In-Data-Review    
Varying morphological features in many groups of tropical vines confound identification, requiring molecular tools for distinguishing species. Confusion is amplified in Psiguria, a small genus found in Central and South America and the Caribbean, because male and female flowers of these monoecious plants are widely separated by time and position on a branch. We present the first phylogeny of Psiguria utilizing a combination of eight chloroplast intergenic spacers, the internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA repeat, and the intron of the low-copy nuclear gene serine/threonine phosphatase, for a total aligned length of 9456 base pairs. Analyses include multiple accessions of all species in the genus. The data support the monophyly of Psiguria and elucidate several species boundaries. Also presented are Psiguria-specific DNA barcodes, which include the chloroplast regions: ndhC-trnV, rps16-trnQ, rpoB-trnC, ndhF-rpl32, and psbZ-trnM. For the first time, systematists, ecologists, and evolutionary biologists will have the tools to confidently identify species of Psiguria with DNA barcodes that may be useful in other genera of Cucurbitaceae.
P Roxanne Steele; Lauren M Friar; Lawrence E Gilbert; Robert K Jansen
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Publication Detail:
Type:  Journal Article     Date:  2009-12-18
Journal Detail:
Title:  American journal of botany     Volume:  97     ISSN:  0002-9122     ISO Abbreviation:  Am. J. Bot.     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2011-05-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370467     Medline TA:  Am J Bot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  156-73     Citation Subset:  -    
Section of Integrative Biology, The University of Texas at Austin, 1 University Station C0930, Austin, Texas 78712 USA.
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