Document Detail

Molecular surveillance of shiga toxigenic Escherichia coli O157 by PulseNet USA.
MedLine Citation:
PMID:  16161697     Owner:  NLM     Status:  MEDLINE    
PulseNet USA is the national molecular subtyping network system for foodborne disease surveillance. Sixty-four public health and food regulatory laboratories participate in PulseNet USA and routinely perform pulsed-field gel electrophoresis of Shiga toxigenic Escherichia coli isolated from humans, food, water, and the environment on a real-time basis. Clusters of infection are detected in three ways within this system: through rapidly alerting the participants in the electronic communication forum, the PulseNet Web conference; through cluster analysis by the database administrators at the coordinating center at the Centers for Disease Control and Prevention of the patterns uploaded to the central server by the participants; and by matching profiles of strains from nonhuman sources with recent human uploads to the national server. The strengths, limitations, and scope for future improvements of PulseNet are discussed with examples from 2002. In that year, notices of 30 clusters of Shiga toxigenic E. coli O157 infections were posted on the Web conference, 26 of which represented local outbreaks, whereas four were multistate outbreaks. Another 27 clusters were detected by central cluster detection performed at the Centers for Disease Control and Prevention, of which five represented common source outbreaks confirmed after finding an isolate with the outbreak pattern in the implicated food. Ten food isolates submitted without suspicion of an association to human disease matched human isolates in the database, and an epidemiologic link to human cases was established for six of them.
Peter Gerner-Smidt; Jennifer Kincaid; Kristy Kubota; Kelley Hise; Susan B Hunter; Mary-Ann Fair; Dawn Norton; Ann Woo-Ming; Terry Kurzynski; Mark J Sotir; Marcus Head; Kristin Holt; Bala Swaminathan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of food protection     Volume:  68     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-09-15     Completed Date:  2005-10-06     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1926-31     Citation Subset:  IM    
Centers for Disease Control and Prevention, National Center of Infectious Diseases, Division of Bacterial and Mycotic Diseases, Foodborne and Diarrheal Diseases Branch, Atlanta, Georgia 30333, USA.
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MeSH Terms
Bacterial Typing Techniques*
Cluster Analysis
Databases as Topic
Disease Outbreaks
Electrophoresis, Gel, Pulsed-Field / methods*
Escherichia coli O157 / classification,  isolation & purification*
Food Microbiology
Information Services*
Population Surveillance
Public Health*
Quality Control
United States

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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