| Molecular studies on the ecology of Listeria monocytogenes in the smoked fish processing industry. | |
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MedLine Citation:
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PMID: 11133446 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have applied molecular approaches, including PCR-based detection strategies and DNA fingerprinting methods, to study the ecology of Listeria monocytogenes in food processing environments. A total of 531 samples, including raw fish, fish during the cold-smoking process, finished product, and environmental samples, were collected from three smoked fish processing facilities during five visits to each facility. A total of 95 (17.9%) of the samples tested positive for L. monocytogenes using a commercial PCR system (BAX for Screening/Listeria monocytogenes), including 57 (27.7%) environmental samples (n = 206), 8 (7.8%) raw material samples (n = 102), 23 (18.1%) samples from fish in various stages of processing(n = 127), and 7 (7.3%) finished product samples (n = 96). L. monocytogenes was isolated from 85 samples (16.0%) using culture methods. Used in conjunction with a 48-h enrichment in Listeria Enrichment Broth, the PCR system had a sensitivity of 91.8% and a specificity of 96.2%. To track the origin and spread of L. monocytogenes, isolates were fingerprinted by automated ribotyping. Fifteen different ribotypes were identified among 85 isolates tested. Ribotyping data established possible contamination patterns, implicating raw materials and the processing environment as potential sources of finished product contamination. Analysis of the distribution of ribotypes revealed that each processing facility had a unique contamination pattern and that specific ribotypes persisted in the environments of two facilities over time (P < or = 0.0006). We conclude that application of molecular approaches can provide critical information on the ecology of different L. monocytogenes strains in food processing environments. This information can be used to develop practical recommendations for improved control of this important food-borne pathogen in the food industry. |
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Authors:
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D M Norton; M A McCamey; K L Gall; J M Scarlett; K J Boor; M Wiedmann |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Applied and environmental microbiology Volume: 67 ISSN: 0099-2240 ISO Abbreviation: Appl. Environ. Microbiol. Publication Date: 2001 Jan |
Date Detail:
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Created Date: 2001-01-19 Completed Date: 2001-05-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7605801 Medline TA: Appl Environ Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 198-205 Citation Subset: IM |
Affiliation:
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Food Safety Laboratory, Department of Food Science, Cornell University, Ithaca, New York 14853, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Ecology Environmental Monitoring / methods* Fishes* / microbiology Food-Processing Industry* Humans Listeria Infections / microbiology, transmission Listeria monocytogenes / classification, genetics*, isolation & purification* Polymerase Chain Reaction Ribotyping |
| Comments/Corrections | |
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