| Molecular structure of human topoisomerase II alpha revealed by atomic force microscopy. | |
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MedLine Citation:
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PMID: 9972548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The entire human topoisomerase II alpha (hTopoII alpha) dimer was expressed in the yeast Saccaromyces cerevisiae, purified to homogeneity, and subjected to atomic force microscopy (AFM) under a tapping mode. Molecular images obtained exhibited a 'heart or donut-like' structure with a large axial hole. The main benefit of the application of AFM to study the hTopoII alpha is that clear images of the internal 'pore' have been achieved without crystallization, staining, or fixation of the sample. These images are consistent with the model in which topoisomerase II has a large internal gate for DNA strand passage. |
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Authors:
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S R Nettikadan; C S Furbee; M T Muller; K Takeyasu |
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Publication Detail:
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Type: Letter; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of electron microscopy Volume: 47 ISSN: 0022-0744 ISO Abbreviation: J Electron Microsc (Tokyo) Publication Date: 1998 |
Date Detail:
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Created Date: 1999-03-25 Completed Date: 1999-03-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7611157 Medline TA: J Electron Microsc (Tokyo) Country: JAPAN |
Other Details:
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Languages: eng Pagination: 671-4 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, Neoplasm DNA Topoisomerases, Type II / chemistry*, isolation & purification, ultrastructure DNA Topoisomerases, Type II, Eukaryotic* DNA-Binding Proteins Humans Isoenzymes / chemistry*, isolation & purification, ultrastructure Microscopy, Atomic Force / methods* Models, Molecular Protein Conformation Saccharomyces cerevisiae / enzymology |
| Grant Support | |
ID/Acronym/Agency:
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2P30CA16058/CA/NCI NIH HHS; GM 44373/GM/NIGMS NIH HHS; U01-CA63185/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Neoplasm; 0/DNA-Binding Proteins; 0/Isoenzymes; EC 5.99.1.-/DNA Topoisomerases, Type II, Eukaryotic; EC 5.99.1.3/DNA Topoisomerases, Type II; EC 5.99.1.3/DNA topoisomerase II alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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