| Molecular structure and function of rat platelet-derived growth factor beta-receptor gene promoter. | |
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MedLine Citation:
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PMID: 9797189 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To understand the regulatory mechanism of platelet-derived growth factor beta-receptor gene expression. METHODS: A 1.7 kb genomic fragment was obtained from a rat genomic library. After we had determined an entire sequence of this fragment, transcription start sites were determined both by primer extension analysis and by riboprobe mapping. We performed a functional promoter assay by using a dual-luciferase reporter system. Progressive 5'-deletions of the fragment and site-directed mutagenesis for the CCAAT motif located at -67 or -94 were used for the assay, and their promoter activities in vascular smooth muscle cells were assessed. Gel-mobility shift analysis was also performed for the CCAAT motif at -67. Effects of the upstream sequence spanning -310 through -120 on heterologous gene promoters were also investigated. RESULTS: Multiple transcription start sites were observed in the 5'-flanking region, and the 1.7 kb sequence was actually active as a functional promoter in vascular smooth muscle cells. Two important sequences responsible for the basal transcriptional activity were identified by the functional promoter assay. One was the CCAAT motif at -67 which acts as a promoter itself, and the other was the upstream region spanning -310 through -210 which positively regulates the basal promoter activity. CONCLUSION: The basal promoter activity of the rat platelet-derived growth factor beta-receptor gene is mainly regulated by the interaction or coordination of two sequences, the CCAAT motif and the upstream control element. |
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Authors:
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Y Kitami; T Fukuoka; T Okura; Y Takata; M Maguchi; M Igase; K Kohara; K Hiwada |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of hypertension Volume: 16 ISSN: 0263-6352 ISO Abbreviation: J. Hypertens. Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1999-01-04 Completed Date: 1999-01-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8306882 Medline TA: J Hypertens Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 437-45 Citation Subset: IM |
Affiliation:
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The Second Department of Internal Medicine, Ehime University School of Medicine, Japan. kitamiyk@m.ehime-u.ac.jp |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U44943 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cells, Cultured Gene Expression Regulation Genome Male Molecular Sequence Data Muscle, Smooth, Vascular / physiology Promoter Regions, Genetic / genetics* Rats Rats, Sprague-Dawley Receptor, Platelet-Derived Growth Factor beta Receptors, Platelet-Derived Growth Factor / genetics* Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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EC 2.7.10.1/Receptor, Platelet-Derived Growth Factor beta; EC 2.7.10.1/Receptors, Platelet-Derived Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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