Document Detail

Molecular sieving of hyaluronan by synovial interstitial matrix and lymphatic capillary endothelium evaluated by lymph analysis in rabbits.
MedLine Citation:
PMID:  14609529     Owner:  NLM     Status:  MEDLINE    
Synovial fluid hyaluronan (HA) profoundly buffers fluid loss from joints. This is attributed to the osmotic pressure of HA reflected by the joint lining. The aims were to quantify HA sieving during fluid drainage from joint to lymphatics and to compare the contributions of the synovial lining and lymphatic endothelium to sieving. HA (2100 kDa) and fluorescein-dextran (FD, 20 kDa) were infused under constant pressure into the knee cavity in anaesthetised rabbits. Samples were taken of femoral lymph and, after approximately 3-h transsynovial filtration, subsynovial fluid and mixed intra-articular fluid. [HA] and [FD] were analysed by HPLC and reflection was calculated as one minus transmitted fraction. Subsynovial and lymph [HA] were 16 and 12% of intra-articular [HA], which increased to 2.6 times infusate [HA] (P < 0.001, ANOVA, n = 19). [FD] was not significantly changed in infusate, aspirate, and subsynovial fluid but fell to 62% in femoral lymph due to dilution by skin/muscle lymph. The HA reflected fraction for the cavity-to-lymph barrier, R(lymph), was 0.54 +/- 0.03 (n = 82, mean +/- SEM), compared with 0.51 +/- 0.07 for cavity-to-subsynovium (R(syn), P > 0.05, Bonferroni) and 0.07 +/- 0.18 for subynovium-to-lymph (R(endo), P < 0.0001, Bonferroni). Lymphatic capillary endothelial reflection R(endo) was not significantly different from zero (one-sample t test). It is concluded that HA is partially sieved out of fluid leaving the joint cavity, and the sieve is the synovial lining interstitial matrix, not lymphatic capillary endothelium.
S Sabaratnam; R M Mason; J R Levick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Microvascular research     Volume:  66     ISSN:  0026-2862     ISO Abbreviation:  Microvasc. Res.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-11-11     Completed Date:  2004-07-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0165035     Medline TA:  Microvasc Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  227-36     Citation Subset:  IM    
Department of Physiology, St. George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, UK.
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MeSH Terms
Chromatography, High Pressure Liquid
Dextrans / pharmacology
Endothelium, Lymphatic / pathology*
Femur / pathology
Fluoresceins / pharmacology
Hyaluronic Acid / pharmacology*
Knee Joint / pathology
Lymph / metabolism*
Lymphatic Vessels / pathology*
Models, Theoretical
Osmotic Pressure
Synovial Membrane / drug effects,  pathology*
Reg. No./Substance:
0/Fluoresceins; 0/fluorescein-dextran; 9004-54-0/Dextrans; 9004-61-9/Hyaluronic Acid
Erratum In:
Microvasc Res. 2004 May 27;67(3):245-6

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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