Document Detail

Molecular scissors for in situ cellular repair.
MedLine Citation:
PMID:  22283548     Owner:  NLM     Status:  Publisher    
The engineering of protein-DNA interactions in different protein scaffolds may provide "toolkits" to modify the genome. Homing endonucleases are powerful tools for genome manipulation through homologous recombination, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. Therefore, the combination of a precise "cutter" with the presence of a natural or modified homologous DNA donor provides a potentially useful means to modify the genome. However, the basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. The engineering of homing endonucleases and alternative scaffolds, such as zinc fingers or transcription activator-like effector domains, has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Customized homing endonucleases targeting selected human genes can excise or correct regions of genes implicated in monogenic diseases, thereby representing important tools for intervention in eukaryotic genomes.
Jesús Prieto; Rafael Molina; Guillermo Montoya
Related Documents :
12956058 - Comparison between the interactions of adenovirus-derived peptides with plasmid dna and...
22363758 - Two cellular protein kinases, dna-pk and pka, phosphorylate the adenoviral l4-33k prote...
18693688 - Time-resolved fluorescence spectroscopy reveals functional differences of cationic poly...
9090708 - Self-assembling dna-lipid particles for gene transfer.
9726918 - Structural studies of a stable parallel-stranded dna duplex incorporating isoguanine:cy...
6154928 - Native ribonucleoprotein is an efficient transcriptional complex of avian myeloblastosi...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-28
Journal Detail:
Title:  Critical reviews in biochemistry and molecular biology     Volume:  -     ISSN:  1549-7798     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903774     Medline TA:  Crit Rev Biochem Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Macromolecular Crystallography Group, Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre (CNIO) , Melchor Fdez Almagro, Madrid , Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Structures of A?17-42 Trimers in Isolation and with Five Small-Molecule Drugs Using a Hierarchical C...
Next Document:  Selective Palladium-Catalyzed C-F Activation/Carbon-Carbon Bond Formation of Polyfluoroaryl Oxazolin...