Document Detail


Molecular scissors for in situ cellular repair.
MedLine Citation:
PMID:  22283548     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The engineering of protein-DNA interactions in different protein scaffolds may provide "toolkits" to modify the genome. Homing endonucleases are powerful tools for genome manipulation through homologous recombination, as these enzymes possess a very low frequency of DNA cleavage in eukaryotic genomes due to their high specificity. Therefore, the combination of a precise "cutter" with the presence of a natural or modified homologous DNA donor provides a potentially useful means to modify the genome. However, the basis of protein-DNA recognition must be understood to generate tailored enzymes that target the DNA at sites of interest. The engineering of homing endonucleases and alternative scaffolds, such as zinc fingers or transcription activator-like effector domains, has demonstrated the potential of these approaches to create new specific instruments to target genes for inactivation or repair. Customized homing endonucleases targeting selected human genes can excise or correct regions of genes implicated in monogenic diseases, thereby representing important tools for intervention in eukaryotic genomes.
Authors:
Jesús Prieto; Rafael Molina; Guillermo Montoya
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-1-28
Journal Detail:
Title:  Critical reviews in biochemistry and molecular biology     Volume:  -     ISSN:  1549-7798     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-1-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8903774     Medline TA:  Crit Rev Biochem Mol Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Macromolecular Crystallography Group, Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre (CNIO) , Melchor Fdez Almagro, Madrid , Spain.
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