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Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii).
MedLine Citation:
PMID:  23415331     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Acyl-CoA binding proteins (ACBPs) have been identified in most branches of life, and play various roles in lipid metabolism, among other functions. Plants contain multiple classes of ACBP genes. The most diverse group is the class III proteins. Tung tree (Vernicia fordii) contains two such genes, designated VfACBP3A and VfACBP3B. The two proteins are significantly different in length and sequence. Analysis of tung ACBP3 genes revealed significant evolution, suggesting relatively ancient divergence of the two genes from a common ancestor. Phylogenetic comparisons of multiple plant class III proteins suggest that this group is the most evolutionarily dynamic class of ACBP. Both tung ACBP3 genes are expressed at similar levels in most tissues tested, but ACBP3A is stronger in leaves. Three-dimensional modeling predictions confirmed the presence of the conserved four α-helix bundle acyl-CoA binding (ACB); however, other regions of these proteins likely fold much differently. Acyl-CoA binding assays revealed different affinities for different acyl-CoAs, possibly contradicting the redundancy of function suggested by the gene expression studies. Subcellular targeting of transiently-expressed plant ACBP3 proteins contradicted earlier studies, and suggested that at least some class III ACBPs may be predominantly targeted to endoplasmic reticulum membranes, with little or no targeting to the apoplast.
Authors:
Steven Pastor; Kandan Sethumadhavan; Abul H J Ullah; Satinder Gidda; Heping Cao; Catherine Mason; Dorselyn Chapital; Brian Scheffler; Robert Mullen; John Dyer; Jay Shockey
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-9
Journal Detail:
Title:  Plant science : an international journal of experimental plant biology     Volume:  203-204C     ISSN:  1873-2259     ISO Abbreviation:  Plant Sci.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-2-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882015     Medline TA:  Plant Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  79-88     Citation Subset:  -    
Copyright Information:
Published by Elsevier Ireland Ltd.
Affiliation:
Southern Regional Research Center, United States Department of Agriculture-Agricultural Research Service, 1100 Robert E. Lee Blvd., New Orleans, LA 70124, USA.
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