Document Detail


Molecular profiling of hematopoietic stem cells.
MedLine Citation:
PMID:  17666739     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Gene expression profiling using microarrays is a powerful method for studying the biology of hematopoietic stem cells (HSCs). Here, we present methods for activating HSCs with the chemotherapeutic drug 5-Fluorouracil, isolating HSCs from whole bone marrow, and performing microarray analysis. We also discuss quality control criteria for identifying good arrays and bioinformatics strategies for analyzing them. Using these methods, we have characterized the gene expression signatures of HSC quiescence and proliferation and have constructed a molecular model of HSC activation and self-renewal.
Authors:
Teresa V Bowman; Akil A Merchant; Margaret A Goodell
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  134     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2007  
Date Detail:
Created Date:  2007-08-01     Completed Date:  2007-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  IM    
Affiliation:
Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calibration
Cell Separation / methods
Cells, Cultured
Fluorouracil / pharmacology
Gene Expression Profiling / methods*
Hematopoietic Stem Cells / drug effects,  metabolism*
Mice
Mice, Inbred C57BL
Oligonucleotide Array Sequence Analysis / methods*,  standards
Quality Control
RNA, Messenger / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/RNA, Messenger; 51-21-8/Fluorouracil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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