Document Detail

Molecular profiling of hematopoietic stem cells.
MedLine Citation:
PMID:  17666739     Owner:  NLM     Status:  MEDLINE    
Gene expression profiling using microarrays is a powerful method for studying the biology of hematopoietic stem cells (HSCs). Here, we present methods for activating HSCs with the chemotherapeutic drug 5-Fluorouracil, isolating HSCs from whole bone marrow, and performing microarray analysis. We also discuss quality control criteria for identifying good arrays and bioinformatics strategies for analyzing them. Using these methods, we have characterized the gene expression signatures of HSC quiescence and proliferation and have constructed a molecular model of HSC activation and self-renewal.
Teresa V Bowman; Akil A Merchant; Margaret A Goodell
Related Documents :
18817319 - Differential gene expression of high and low immune responder canadian holstein dairy c...
12882789 - Analysis of pseudomonas aeruginosa corneal infection using an oligonucleotide microarray.
15136399 - Gene expression profiling identifies matriptase overexpression in malignant mesothelioma.
16098629 - Multievidence microarray mining.
12915489 - Transcriptional response of lymphoblastoid cells to ionizing radiation.
18807069 - Gene expression changes induced by space flight in single-cells of the fern ceratopteri...
8316859 - Regulation of lymphoid-specific immunoglobulin mu heavy chain gene enhancer by ets-doma...
22419659 - Screening and identification of distant metastasis-related differentially expressed gen...
15550939 - The evolution of chronic infection strategies in the alpha-proteobacteria.
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  134     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2007  
Date Detail:
Created Date:  2007-08-01     Completed Date:  2007-10-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-16     Citation Subset:  IM    
Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Separation / methods
Cells, Cultured
Fluorouracil / pharmacology
Gene Expression Profiling / methods*
Hematopoietic Stem Cells / drug effects,  metabolism*
Mice, Inbred C57BL
Oligonucleotide Array Sequence Analysis / methods*,  standards
Quality Control
RNA, Messenger / isolation & purification
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/RNA, Messenger; 51-21-8/Fluorouracil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Brachial plexus blocks: a review of approaches and techniques.
Next Document:  Methods for imaging cell fates in hematopoiesis.