| Molecular physiology of adventitious root formation in Petunia hybrida cuttings: involvement of wound response and primary metabolism. | |
| | |
MedLine Citation:
|
PMID: 19076299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Adventitious root formation (ARF) in the model plant Petunia hybrida cv. Mitchell has been analysed in terms of anatomy, gene expression, enzymatic activities and levels of metabolites. This study focuses on the involvement of wound response and primary metabolism. Microscopic techniques were complemented with targeted transcript, enzyme and metabolite profiling using real time polymerase chain reaction (PCR), Northern blot, enzymatic assays, chromatography and mass spectrometry. Three days after severance from the stock plants, first meristematic cells appeared which further developed into root primordia and finally adventitious roots. Excision of cuttings led to a fast and transient increase in the wound-hormone jasmonic acid, followed by the expression of jasmonate-regulated genes such as cell wall invertase. Analysis of soluble and insoluble carbohydrates showed a continuous accumulation during ARF. A broad metabolite profiling revealed a strong increase in organic acids and resynthesis of essential amino acids. Substantial changes in enzyme activities and metabolite levels indicate that specific enzymes and metabolites might play a crucial role during ARF. Three metabolic phases could be defined: (i) sink establishment phase characterized by apoplastic unloading of sucrose and being probably mediated by jasmonates; (ii) recovery phase; and (iii) maintenance phase, in which a symplastic unloading occurs. |
| | |
Authors:
|
Amir H Ahkami; Sandra Lischewski; Klaus-T Haensch; Svetlana Porfirova; Joerg Hofmann; Hardy Rolletschek; Michael Melzer; Philipp Franken; Bettina Hause; Uwe Druege; Mohammad R Hajirezaei |
Related Documents
:
|
3548429 - Effects of d-amino acid substituents on degradation of lhrh analogues by proximal tubule. 17769759 - Light and hormones: interchangeability in the induction of nitrate reductase. 2046209 - Gnrh analogs in reproductive medicine. 12414809 - Structural divergence of human ghrelin. identification of multiple ghrelin-derived mole... 3129279 - Immunological studies on teleocalcin and salmon corpuscles of stannius. 8886299 - Effect of acute tryptophan depletion on behavioral, cardiovascular, and hormonal sensit... 7810629 - Effect of hyperinsulinemia and hyperaminoacidemia on muscle and liver protein synthesis... 15217279 - Cytotoxic triterpenoids from acridocarpus vivy from the madagascar rain forest. 7243199 - Ascorbic acid in the treatment of alkali burns of the eye. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-12-05 |
Journal Detail:
|
Title: The New phytologist Volume: 181 ISSN: 1469-8137 ISO Abbreviation: New Phytol. Publication Date: 2009 |
Date Detail:
|
Created Date: 2009-01-21 Completed Date: 2009-03-16 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9882884 Medline TA: New Phytol Country: England |
Other Details:
|
Languages: eng Pagination: 613-25 Citation Subset: IM |
Affiliation:
|
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acids
/
metabolism Carbohydrate Metabolism / genetics Cell Respiration Citric Acid Cycle Cyclopentanes / metabolism Fatty Acids, Unsaturated / metabolism Gene Expression Regulation, Plant Genes, Plant Glycolysis Oxylipins / metabolism Petunia / cytology, enzymology, genetics, metabolism* Plant Proteins / genetics, metabolism Plant Roots / cytology, enzymology, genetics, metabolism* RNA, Messenger / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acids; 0/Cyclopentanes; 0/Fatty Acids, Unsaturated; 0/Oxylipins; 0/Plant Proteins; 0/RNA, Messenger; 67204-66-4/12-oxophytodienoic acid; 6894-38-8/jasmonic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: C1 metabolism and chlorophyll synthesis: the Mg-protoporphyrin IX methyltransferase activity is depe...
Next Document: Long-chain polyunsaturated fatty acids in a diabetic teenager during and after nine repeated episode...