Document Detail


Molecular physiology of adventitious root formation in Petunia hybrida cuttings: involvement of wound response and primary metabolism.
MedLine Citation:
PMID:  19076299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Adventitious root formation (ARF) in the model plant Petunia hybrida cv. Mitchell has been analysed in terms of anatomy, gene expression, enzymatic activities and levels of metabolites. This study focuses on the involvement of wound response and primary metabolism. Microscopic techniques were complemented with targeted transcript, enzyme and metabolite profiling using real time polymerase chain reaction (PCR), Northern blot, enzymatic assays, chromatography and mass spectrometry. Three days after severance from the stock plants, first meristematic cells appeared which further developed into root primordia and finally adventitious roots. Excision of cuttings led to a fast and transient increase in the wound-hormone jasmonic acid, followed by the expression of jasmonate-regulated genes such as cell wall invertase. Analysis of soluble and insoluble carbohydrates showed a continuous accumulation during ARF. A broad metabolite profiling revealed a strong increase in organic acids and resynthesis of essential amino acids. Substantial changes in enzyme activities and metabolite levels indicate that specific enzymes and metabolites might play a crucial role during ARF. Three metabolic phases could be defined: (i) sink establishment phase characterized by apoplastic unloading of sucrose and being probably mediated by jasmonates; (ii) recovery phase; and (iii) maintenance phase, in which a symplastic unloading occurs.
Authors:
Amir H Ahkami; Sandra Lischewski; Klaus-T Haensch; Svetlana Porfirova; Joerg Hofmann; Hardy Rolletschek; Michael Melzer; Philipp Franken; Bettina Hause; Uwe Druege; Mohammad R Hajirezaei
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-05
Journal Detail:
Title:  The New phytologist     Volume:  181     ISSN:  1469-8137     ISO Abbreviation:  New Phytol.     Publication Date:  2009  
Date Detail:
Created Date:  2009-01-21     Completed Date:  2009-03-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882884     Medline TA:  New Phytol     Country:  England    
Other Details:
Languages:  eng     Pagination:  613-25     Citation Subset:  IM    
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism
Carbohydrate Metabolism / genetics
Cell Respiration
Citric Acid Cycle
Cyclopentanes / metabolism
Fatty Acids, Unsaturated / metabolism
Gene Expression Regulation, Plant
Genes, Plant
Glycolysis
Oxylipins / metabolism
Petunia / cytology,  enzymology,  genetics,  metabolism*
Plant Proteins / genetics,  metabolism
Plant Roots / cytology,  enzymology,  genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Cyclopentanes; 0/Fatty Acids, Unsaturated; 0/Oxylipins; 0/Plant Proteins; 0/RNA, Messenger; 67204-66-4/12-oxophytodienoic acid; 6894-38-8/jasmonic acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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