Document Detail

Molecular and physiological comparison of spoilage wineyeasts.
MedLine Citation:
PMID:  23311591     Owner:  NLM     Status:  Publisher    
AIMS: Dekkerabruxellensis and Pichia guilliermondii are contaminating yeasts in wine due to the production of phenolic aromas. Although the degradation pathway of cinnamic acids, precursors of these phenolic compounds has been described in D. bruxellensis, no such pathway has been described in P. guilliermondii. METHODS AND RESULTS: A molecular and physiological characterization of 14 D. bruxellensis and 15 P. guilliermondiiphenol-producing strainswas carried out. Bothp-coumarate decarboxylase (CD) and vinyl reductase (VR) activities, responsible for the production of volatile phenols, were quantified and the production of 4-VP and 4-EP weremeasured. All D. bruxellensis and some P. guilliermondiistrains showed the two enzymatic activities, whilst 11 of the 15 strains of this latter species showed only CD activity and did not produce 4-EP in the assay conditions.Furthermore, PCR products obtained with degenerated primers showed a low homology with the sequence of the gene for a phenyl acrylic acid decarboxylase activitydescribed in Saccharomyces cerevisiae CONCLUSIONS: D. bruxellensis and P. guilliermondii may share a similar metabolic pathway for the degradation of cinnamic acids. © 2013 The Authors Journal of Applied Microbiology © 2013 The Society for Applied Microbiology.
M P Sangorrín; V García; C A Lopes; J S Sáez; C Martínez; M A Ganga
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-14
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Authors Journal of Applied Microbiology © 2013 The Society for Applied Microbiology.
Grupo de Biodiversidad y Biotecnología de Levaduras, Instituto Multidisciplinario de Investigación y Desarrollo de la Patagonia Norte (IDEPA, CONICET-UNCo), Dpto. de Química, Facultad de Ingeniería, Universidad Nacional del Comahue, Buenos Aires 1400, (8300), Neuquén, Argentina.
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