Document Detail

Molecular phylogeny of methylotrophs in a deep-sea sediment from a tropical west Pacific Warm Pool.
MedLine Citation:
PMID:  19712348     Owner:  NLM     Status:  MEDLINE    
The presence and phylogeny of methylotrophs, including methanotrophs, in a deep-sea sediment of a tropical west Pacific Warm Pool site WP was investigated by molecular marker-based analysis of mxaF, pmoA and archaeal 16S rRNA genes. MxaF amino acid sequence analysis revealed that microbes belonging to the alpha-Proteobacteria and most related to Hyphomicrobium and Methylobacterium were the dominant aerobic methylotrophs in this deep-sea sediment; also, a small percentage of type II methanotrophs, closely related to Methylocystis and Methylosinus, were detected in this environment. On the other hand, the use of a pmoA gene marker could not demonstrate the presence of any methanotrophs in this environment, suggesting that the mxaF gene probe is a more suitable marker in this deep-sea sediment for the detection of methylotrophs (including methanotrophs). mxaF quantitative polymerase chain reaction results showed that the west Pacific WP sediment contained approximately 3x10(4-5) methylotrophs per gram sediment, 10-100 times more than the samples collected from several other deep-sea Pacific sediments, but, on the other hand, about 10 times less than the amounts present in samples collected from rice and flower garden soil. Archaeal diversity as analyzed by 16S rRNA gene sequences indicated that a non-thermophilic marine group I crenarchaeote was the major archaeal group present in the west Pacific WP.
Peng Wang; Fengping Wang; Meixiang Xu; Xiang Xiao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS microbiology ecology     Volume:  47     ISSN:  1574-6941     ISO Abbreviation:  FEMS Microbiol. Ecol.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2009-08-28     Completed Date:  2009-09-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8901229     Medline TA:  FEMS Microbiol Ecol     Country:  England    
Other Details:
Languages:  eng     Pagination:  77-84     Citation Subset:  IM    
Key Laboratory of Marine Biogenetic Resources, State Oceanic Administration, Xiamen, PR China.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ561090;  AJ561091;  AJ561092;  AJ561093;  AJ561094;  AJ561095;  AJ561096;  AJ561097;  AJ561098;  AJ561099;  AJ561100;  AJ561101;  AJ561102;  AJ561103;  AJ561104;  AJ561105;  AJ561106;  AJ561107;  AJ561108
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MeSH Terms
Alphaproteobacteria / classification,  genetics*,  isolation & purification,  metabolism
Bacterial Proteins / genetics
Crenarchaeota* / classification,  genetics,  isolation & purification,  metabolism
DNA, Archaeal / analysis
DNA, Bacterial / analysis
Geologic Sediments / microbiology*
Methane / metabolism*
Molecular Sequence Data
Pacific Ocean
RNA, Ribosomal, 16S / genetics
Seawater / microbiology*
Sequence Analysis, DNA
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA, Archaeal; 0/DNA, Bacterial; 0/RNA, Ribosomal, 16S; 74-82-8/Methane

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