Document Detail


Molecular and phylogenetic analysis of human influenza virus among Iranian patients in Shiraz, Iran.
MedLine Citation:
PMID:  17457914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Influenza is a viral respiratory pathogen responsible for frequent seasonal epidemics. There are currently three major human influenza viruses in global circulation namely A/H1N1, A/H3N2, and B. The objective of this study was to determine the human influenza virus genotypes in Shiraz, the capital of the Fars province of Iran. Three hundred patients suspected with human influenza virus infection were enrolled in this survey (2004-2005). The throat samples were cultured and titrated by hemagglutination (HA) assay. Typing and subtyping were performed by an in-house developed multiplex RT-PCR. Moreover, the phylogenetic analysis was carried out for HA gene. A total of 24 samples were found to be positive for human influenza virus infection, 17 H1N1 and 7 H3N2. These results were in agreement with the HI assay. The phylogenetic analysis results revealed that the Iranian H1N1 isolated were close to the A/New Caledonia/20/99 vaccine strain genetically and the Iranian H3N2 isolates were also related closely to the Fujian/411/021 and California/7/2004 vaccine strains. However, a slight genetic drift was found in these isolates. In conclusion, it was demonstrated that both influenza A subtypes A/H1N1 and A/H3N2 were dominant among Iranian patients in Shiraz during the 2004/5 winter season.
Authors:
Mahsa Shahidi; Masoumeh Tavassoti Kheiri; Samad Amini-Bavil-Olyaee; Masoud Hosseini; Afagh Moattari; Mansoureh Tabatabaeian; Rouzbeh Bashar; Ramin Sarrami-Forooshani; Fereidoun Mahboudi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical virology     Volume:  79     ISSN:  0146-6615     ISO Abbreviation:  J. Med. Virol.     Publication Date:  2007 Jun 
Date Detail:
Created Date:  2007-06-04     Completed Date:  2007-07-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7705876     Medline TA:  J Med Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  803-10     Citation Subset:  IM    
Copyright Information:
(c) 2007 Wiley-Liss, Inc.
Affiliation:
Influenza Unit, Pasteur Institute of Iran, Tehran, Iran.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/DQ973299;  DQ973300;  DQ973301;  DQ973302;  DQ973303;  DQ973304;  DQ973305;  DQ981739;  DQ981740;  DQ981741;  DQ981742
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Child
Genotype
Hemagglutinin Glycoproteins, Influenza Virus / genetics*
Humans
Influenza A Virus, H1N1 Subtype / classification,  genetics,  isolation & purification
Influenza A Virus, H3N2 Subtype / classification,  genetics,  isolation & purification
Influenza, Human / virology*
Influenzavirus B / classification,  genetics,  isolation & purification
Iran
Middle Aged
Molecular Sequence Data
Orthomyxoviridae / classification*,  genetics*,  isolation & purification
Pharynx / virology
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, Protein
Chemical
Reg. No./Substance:
0/Hemagglutinin Glycoproteins, Influenza Virus

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