| Molecular phenotyping of mannosyltransferases-deficient Candida albicans cells by high-resolution magic angle spinning NMR. | |
| | |
MedLine Citation:
|
PMID: 19218187 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The yeast Candida albicans is an opportunistic pathogen that causes infections in immunocompromised individuals with a high morbidity and mortality levels. Recognition of yeasts by host cells is directly mediated by cell wall components of the yeast, including a wide range of abundantly expressed glycoconjugates. Of particular interest in C. albicans are the beta-mannosylated epitopes that show a complex expression pattern on N-glycan moiety of phosphopeptidomannans and are absent in the non-pathogenic species Saccharomyces cerevisiae. Being known as potent antigens for the adaptive immune response and elicitors of specific infection-protective antibodies, the exact delineation of beta-mannosides regulation and expression pathways has lately become a major milestone toward the comprehension of host-pathogen interplay. Using the newly developed HR-MAS NMR methodology, we demonstrate the possibility of assessing the general profiles of cell-surface-exposed glycoconjugates from intact living yeast cells without any prior purification step. This technique permitted to directly observe structural modifications of surface expressed phosphodiester-linked beta-mannosides on a series of deletion strains in beta-mannosyltransferases and phospho-mannosyltransferases compared with their parental strains. |
| | |
Authors:
|
Emmanuel Maes; Céline Mille; Xavier Trivelli; Guilhem Janbon; Daniel Poulain; Yann Guérardel |
Related Documents
:
|
15067127 - Illegitimate wnt signaling promotes proliferation of multiple myeloma cells. 19907327 - Regenerating pancreatic beta-cells: plasticity of adult pancreatic cells and the feasib... 2522537 - Targeting and processing of pro-opiomelanocortin in neuronal cell lines. 1743297 - Identification of a 220 kda membrane-associated plant cell protein immunologically rela... 2641367 - A scanning electron microscopic study of exfoliated malignant oral epithelial cells. 3842077 - Histogenesis of anaplastic carcinomas of the thyroid, giant cell variety: immunocytoche... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-01-17 |
Journal Detail:
|
Title: Journal of biochemistry Volume: 145 ISSN: 1756-2651 ISO Abbreviation: J. Biochem. Publication Date: 2009 Apr |
Date Detail:
|
Created Date: 2009-04-06 Completed Date: 2009-07-06 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0376600 Medline TA: J Biochem Country: England |
Other Details:
|
Languages: eng Pagination: 413-9 Citation Subset: IM |
Affiliation:
|
Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576, IFR 147, Université des Sciences et Technologies de Lille 1, 59655, Villeneuve d'Ascq, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Candida albicans
/
cytology*,
enzymology* Magnetic Resonance Spectroscopy Mannans / chemistry, isolation & purification Mannosyltransferases / deficiency* Models, Molecular* Phenotype Phosphopeptides / chemistry, isolation & purification Polysaccharides / chemistry Solubility |
| Chemical | |
Reg. No./Substance:
|
0/Mannans; 0/Phosphopeptides; 0/Polysaccharides; 0/phosphopeptidomannan; EC 2.4.1.-/Mannosyltransferases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Perceived everyday racism, residential segregation, and HIV testing among patients at a sexually tra...
Next Document: Cooperative binding of L-trp to human tryptophan 2,3-dioxygenase: resonance Raman spectroscopic anal...